Literature DB >> 26427680

Measuring Cell-Cell Binding Using Flow-Cytometry.

Zachary C Ruhe1, Christopher S Hayes1,2, David A Low3,4.   

Abstract

Cell-cell adhesion mediates a number of competitive and cooperative microbial interactions. Fluorescence labeling and flow cytometry techniques allow us to observe and measure these interactions rapidly and easily. Here, we describe a method to quantify cell-cell adhesion events between two differentially labeled cell populations.

Keywords:  CdiA; Contact-dependent growth inhibition; Flow cytometry; Fluorescent proteins; Self/nonself recognition; cell–cell binding

Mesh:

Substances:

Year:  2015        PMID: 26427680     DOI: 10.1007/978-1-4939-2871-2_9

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  2 in total

1.  CdiA Effectors from Uropathogenic Escherichia coli Use Heterotrimeric Osmoporins as Receptors to Recognize Target Bacteria.

Authors:  Christina M Beck; Julia L E Willett; David A Cunningham; Jeff J Kim; David A Low; Christopher S Hayes
Journal:  PLoS Pathog       Date:  2016-10-10       Impact factor: 6.823

2.  CdiA Effectors Use Modular Receptor-Binding Domains To Recognize Target Bacteria.

Authors:  Zachary C Ruhe; Josephine Y Nguyen; Jing Xiong; Sanna Koskiniemi; Christina M Beck; Basil R Perkins; David A Low; Christopher S Hayes
Journal:  MBio       Date:  2017-03-28       Impact factor: 7.867

  2 in total

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