BACKGROUND: Polycystic ovary syndrome (PCOS) is characterized by hyperandrogenism, hyperinsulinemia, and infertility. In PCOS, abnormal regulation of relevant genes is required for follicular development. By binding to the 3' untranslated region (3'URT), microRNAs (miRNAs) are widely involved in posttranscriptional gene regulation. However, few studies have been conducted on circulating miRNA expression in PCOS. This study aims to describe altered expression of circulating miR-21 in PCOS. METHODS: The expression of serum miRNAs of PCOS patients were explored using the TaqMan Low Density Array followed by individual quantitative reverse transcription polymerase chain reaction assays. The protein level of LATS1 was determined using Western blot. To validate whether miR-21 targeted LATS1, the luciferase assay was applied. RESULTS: In comparison with normal subjects, the circulating level of miRNA-21 was significantly enhanced in PCOS patients. In PCOS patients, the expression levels of MST1/2, LATS1/2, TAZ were much lower than the control subjects. Luciferase reporter assay revealed that LATS1 was a downstream target of miR-21. CONCLUSIONS: In comparison with normal subjects, serum miR-21 is obviously increased in PCOS patients. Through targeting LATS1, miR-21 could prompt PCOS progression and could act as a novel non-invasive biomarker for diagnosis of PCOS.
BACKGROUND:Polycystic ovary syndrome (PCOS) is characterized by hyperandrogenism, hyperinsulinemia, and infertility. In PCOS, abnormal regulation of relevant genes is required for follicular development. By binding to the 3' untranslated region (3'URT), microRNAs (miRNAs) are widely involved in posttranscriptional gene regulation. However, few studies have been conducted on circulating miRNA expression in PCOS. This study aims to describe altered expression of circulating miR-21 in PCOS. METHODS: The expression of serum miRNAs of PCOSpatients were explored using the TaqMan Low Density Array followed by individual quantitative reverse transcription polymerase chain reaction assays. The protein level of LATS1 was determined using Western blot. To validate whether miR-21 targeted LATS1, the luciferase assay was applied. RESULTS: In comparison with normal subjects, the circulating level of miRNA-21 was significantly enhanced in PCOSpatients. In PCOSpatients, the expression levels of MST1/2, LATS1/2, TAZ were much lower than the control subjects. Luciferase reporter assay revealed that LATS1 was a downstream target of miR-21. CONCLUSIONS: In comparison with normal subjects, serum miR-21 is obviously increased in PCOSpatients. Through targeting LATS1, miR-21 could prompt PCOS progression and could act as a novel non-invasive biomarker for diagnosis of PCOS.
Authors: Anja E Sørensen; Pernille B Udesen; Grzegorz Maciag; Julian Geiger; Negar Saliani; Andrzej S Januszewski; Guozhi Jiang; Ronald C Ma; Anandwardhan A Hardikar; Marie Louise M Wissing; Anne Lis M Englund; Louise T Dalgaard Journal: Front Med (Lausanne) Date: 2019-11-01
Authors: Alexandra E Butler; Vimal Ramachandran; Thozhukat Sathyapalan; Rhiannon David; Nigel J Gooderham; Manasi Benurwar; Soha R Dargham; Shahina Hayat; S Hani Najafi-Shoushtari; Stephen L Atkin Journal: Front Endocrinol (Lausanne) Date: 2020-04-28 Impact factor: 5.555
Authors: Alexandra E Butler; Vimal Ramachandran; Thomas Keith Cunningham; Rhiannon David; Nigel J Gooderham; Manasi Benurwar; Soha R Dargham; Shahina Hayat; Thozhukat Sathyapalan; S Hani Najafi-Shoushtari; Stephen L Atkin Journal: Front Endocrinol (Lausanne) Date: 2020-09-30 Impact factor: 5.555