Literature DB >> 26426961

Correction: Comparative Analysis of Label-Free and 8-Plex iTRAQ Approach for Quantitative Tissue Proteomic Analysis.

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Abstract

Entities:  

Year:  2015        PMID: 26426961      PMCID: PMC4591008          DOI: 10.1371/journal.pone.0139268

Source DB:  PubMed          Journal:  PLoS One        ISSN: 1932-6203            Impact factor:   3.240


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Tables 1 and 5 are incorrectly aligned due to errors introduced in the production process. The publisher apologizes for the errors. Please see the corrected Tables 1 and 5 here.
Table 1

Overview of the number of peptides and the corresponding proteins as being identified in the individual MS-runs.

MethodSample ID# peptide groups# protein groups
LFQ 3_pTa50731096
11_pTa52691099
16_pTa57251185
19_pTa3931937
Mean (SD)_pTa 5000 ± 763 1079 ± 103
9_pT2+53601130
12_pT2+51121136
15_pT2+55161169
17_pT2+54851155
Mean (SD)_pT2+ 5368 ± 184 1148 ± 18
iTRAQ 10 μg1859664
fractionation 60992064
Table 5

Comparison of the quantification results at the peptide and protein level for identifications with conflicting expression trends between fractionated iTRAQ and LFQ

Protein/ PeptidePeptide Log2 Ratio (pT2+ vs. pTa)Protein Log2 Ratio (pT2+ vs. pTa)
Protein NamePeptide sequence used for quantificationiTRAQ + fractionationLFQiTRAQ + fractionationLFQ
Heterochromatin protein 1-binding protein 3 eASYSLIR-0.240.03 -0,30 0.72
mDAILTEAIk-0,370.32
tIPSWATLSASQLAR-0.300.41
SSAVDPEPQVK-1.31
LEDVLPLAFTR-0.24
GASGSFVVVQK-5.12
Actin-related protein 2/3 complex subunit 3 aYLQQLR0.010.61 -0.25 0.37
lIGNMALLPIR-0.43-0.06
lIGNmALLPIR-0.34-
Dolichyl-diphosphooligosaccharide—protein glycosyltransferase subunit 2 sIVEEIEDLVAR0,212.46 -0.06 1.23
eDQVIQLMNAIFSk-0.36-
fELDTSER0.15-
nFESLSEAFSVASAAAVLSHNR-0.12-
qEIQHLFR0.09-
yHVPVVVVPEGSASDTHEQAILR-0.380.62
LQVTNVLSQPLTQATVK-0.33
ISTEVGITNVDLSTVDKDQSIAPK-1.78
NPILWNVADVVIK-3.65
YIANTVELR0.063.15
KH domain-containing, RNA-binding, signal transduction-associated protein 1 KDDEENYLDLFSHK-0.39 -0.25 0.63
ILGPQGNTIK-0.55
DSLDPSFTHAMQLLTAEIEK-2.42
SGSMDPSGAHPSVR0.060.68
qPPLPHR-0.56-
General vesicular transport factor p115 SSQTSGTNEQSSAIVSAR-0.27 -0.10 0.52
SQLNSQSVEITK-0.25
NDGVLLLQALTR-0,232.44
eQDLQLEELR-0.47-
qSEDLGSQFTEIFIk0.31-
vASSTLLDDRR-0.00007-
  1 in total

1.  Comparative Analysis of Label-Free and 8-Plex iTRAQ Approach for Quantitative Tissue Proteomic Analysis.

Authors:  Agnieszka Latosinska; Konstantinos Vougas; Manousos Makridakis; Julie Klein; William Mullen; Mahmoud Abbas; Konstantinos Stravodimos; Ioannis Katafigiotis; Axel S Merseburger; Jerome Zoidakis; Harald Mischak; Antonia Vlahou; Vera Jankowski
Journal:  PLoS One       Date:  2015-09-02       Impact factor: 3.240

  1 in total

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