| Literature DB >> 26425924 |
Linna Wang1, Li Yang1, Li Pan2, Naveen Reddy Kadasala3, Liang Xue1, Robert J Schuster2, Laurie L Parker2, Alexander Wei3,4, W Andy Tao1,2,4.
Abstract
Our understanding of the complex cell entry pathways would greatly benefit from a comprehensive characterization of key proteins involved in this dynamic process. Here we devise a novel proteomic strategy named TITAN (Tracing Internalization and TrAfficking of Nanomaterials) to reveal real-time protein-dendrimer interactions using a systems biology approach. Dendrimers functionalized with photoreactive cross-linkers were internalized by HeLa cells and irradiated at set time intervals, then isolated and subjected to quantitative proteomics. In total, 809 interacting proteins cross-linked with dendrimers were determined by TITAN in a detailed temporal manner during dendrimer internalization, traceable to at least two major endocytic mechanisms, clathrin-mediated and caveolar/raft-mediated endocytosis. The direct involvement of the two pathways was further established by the inhibitory effect of dynasore on dendrimer uptake and changes in temporal profiles of key proteins.Entities:
Mesh：
Substances：
Year: 2015 PMID： 26425924 PMCID： PMC4766814 DOI： 10.1021/jacs.5b07875
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419