| Literature DB >> 26423968 |
John A Calarco1, Ari E Friedland2.
Abstract
The clustered, regularly interspaced, short, palindromic repeat (CRISPR)-associated (CAS) nuclease Cas9 has been used in many organisms to generate specific mutations and transgene insertions. Here we describe a method using the S. pyogenes Cas9 in C. elegans that provides a convenient and effective approach for making heritable changes to the worm genome.Entities:
Keywords: C. elegans; CRISPR; Cas9; Genome editing; Genome engineering
Mesh：
Substances：
Year: 2015 PMID： 26423968 DOI： 10.1007/978-1-4939-2842-2_6
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745