Fabiano Luiz Heggendorn1, Lucio Souza Gonçalves2, Eliane Pedra Dias3, Viviane de Oliveira Freitas Lione4, Márcia Teresa Soares Lutterbach5. 1. Associate Professor, Medical School, Fluminense Federal University; School of Pharmacy, Federal University of Rio de Janeiro, Pharmaceutical Laboratory Bioassays; Laboratory of Biocorrosion and Biodegradation, National Institute of Technology, Rua Feliz da Cunha 11, ap. 806, Tijuca, CEP 20260300, Rio de Janeiro, Brazil, Phone: 55-21-9411-9341, e-mail: fabianohegg@gmail.com. 2. Faculty of Dentistry, Estacio de Sa University, Rio de Janeiro Brazil. 3. Medical School, Fluminense Federal University, Rio de Janeiro, Brazil. 4. School of Pharmacy, Federal University of Rio de Janeiro Pharmaceutical Laboratory Bioassays, Rio de Janeiro, Brazil. 5. Laboratory of Biocorrosion and Biodegradation, National Institute of Technology, Rio de Janeiro, Brazil.
Abstract
AIM: This study assessed the biocorrosive capacity of two bacteria: Desulfovibrio desulfuricans and Desulfovibrio fairfieldensis on endodontic files, as a preliminary step in the development of a biopharmaceutical, to facilitate the removal of endodontic file fragments from root canals. MATERIALS AND METHODS: In the first stage, the corrosive potential of the artificial saliva medium (ASM), modified Postgate E medium (MPEM), 2.5 % sodium hypochlorite (NaOCl) solution and white medium (WM), without the inoculation of bacteria was assessed by immersion assays. In the second stage, test samples were inoculated with the two species of sulphur-reducing bacteria (SRB) on ASM and modified artificial saliva medium (MASM). In the third stage, test samples were inoculated with the same species on MPEM, ASM and MASM. All test samples were viewed under an infinite focus Alicona microscope. RESULTS: No test sample became corroded when immersed only in media, without bacteria. With the exception of one test sample between those inoculated with bacteria in ASM and MASM, there was no evidence of corrosion. Fifty percent of the test samples demonstrated a greater intensity of biocorrosion when compared with the initial assays. CONCLUSION: Desulfovibrio desulfuricans and D. fairfieldensis are capable of promoting biocorrosion of the steel constituent of endodontic files. CLINICAL SIGNIFICANCE: This study describes the initial development of a biopharmaceutical to facilitate the removal of endodontic file fragments from root canals, which can be successfully implicated in endodontic therapy in order to avoiding parendodontic surgery or even tooth loss in such events.
AIM: This study assessed the biocorrosive capacity of two bacteria: Desulfovibrio desulfuricans and Desulfovibrio fairfieldensis on endodontic files, as a preliminary step in the development of a biopharmaceutical, to facilitate the removal of endodontic file fragments from root canals. MATERIALS AND METHODS: In the first stage, the corrosive potential of the artificial saliva medium (ASM), modified Postgate E medium (MPEM), 2.5 % sodium hypochlorite (NaOCl) solution and white medium (WM), without the inoculation of bacteria was assessed by immersion assays. In the second stage, test samples were inoculated with the two species of sulphur-reducing bacteria (SRB) on ASM and modified artificial saliva medium (MASM). In the third stage, test samples were inoculated with the same species on MPEM, ASM and MASM. All test samples were viewed under an infinite focus Alicona microscope. RESULTS: No test sample became corroded when immersed only in media, without bacteria. With the exception of one test sample between those inoculated with bacteria in ASM and MASM, there was no evidence of corrosion. Fifty percent of the test samples demonstrated a greater intensity of biocorrosion when compared with the initial assays. CONCLUSION:Desulfovibrio desulfuricans and D. fairfieldensis are capable of promoting biocorrosion of the steel constituent of endodontic files. CLINICAL SIGNIFICANCE: This study describes the initial development of a biopharmaceutical to facilitate the removal of endodontic file fragments from root canals, which can be successfully implicated in endodontic therapy in order to avoiding parendodontic surgery or even tooth loss in such events.
Authors: Umarevathi Gopalakrishnan; A Sumathi Felicita; Lodd Mahendra; Masroor Ahmed Kanji; Saranya Varadarajan; A Thirumal Raj; Shaikh Mohammed Abdul Feroz; Deepak Mehta; Hosam Ali Baeshen; Shankargouda Patil Journal: Front Bioeng Biotechnol Date: 2021-03-15
Authors: Fabiano Luiz Heggendorn; Aline Guerra Manssour Fraga; Dennis de Carvalho Ferreira; Lucio Souza Gonçalves; Viviane de Oliveira Freitas Lione; Márcia Teresa Soares Lutterbach Journal: Int J Dent Date: 2018-05-10