Ihsan Ates1, Mustafa Kaplan2, Bayram Inan2, Murat Alisik3, Ozcan Erel3, Nisbet Yilmaz2, Serdar Guler4. 1. Ankara Numune Training and Research Hospital, Department of Internal Medicine, Ankara, Turkey. Electronic address: dr.ihsanates@hotmail.com. 2. Ankara Numune Training and Research Hospital, Department of Internal Medicine, Ankara, Turkey. 3. Yıldırım Beyazıt University Medical Faculty, Department of Biochemistry, Ankara, Turkey. 4. Ankara Numune Training and Research Hospital, Department of Endocrinology, Ankara, Turkey.
Abstract
AIMS: Our aim was to examine thiol/disulfide homeostasis, which has a critical role in many cellular activities such as antioxidant protection, detoxification, cell growth and apoptosis, in prediabetic patients. METHODS: The study population was formed of a total of 250 participants; 125 (54 males, 71 females) of which were newly diagnosed with prediabetes, aged over 18 and who had not received any prior treatment and 125 (52 males, 73 females) healthy volunteers. Prediabetic patients were diagnosed using a glucose tolerance test. In both groups, native thiol-disulfide exchanges were examined using the automated measurement method newly developed by Erel and Neselioglu. RESULTS: When compared to the control group, the native thiol (p<0.001) and total thiol (p=0.008) levels, and the native thiol/total thiol (p=0.022) ratio was lower; while disulfide (p=0.001) level and, disulfide/native thiol (p=0.003) and disulfide/total thiol (p=0.022) ratios were higher in prediabetic patients. A positive correlation was determined between disulfide and the fasting blood glucose levels (r=0.394, p=0.017) and glycolysed hemoglobin (HbA1c) (r=0.307, p=0.011). On the other hand, a negative correlation was determined between native thiol and fasting blood glucose levels (r=-0.335, p=0.004). CONCLUSION: With this study, we have shown for the first time that thiol oxidation increases in prediabetic patients and that there is a positive correlation between the disulfide and blood glucose and HbA1c levels.
AIMS: Our aim was to examine thiol/disulfide homeostasis, which has a critical role in many cellular activities such as antioxidant protection, detoxification, cell growth and apoptosis, in prediabeticpatients. METHODS: The study population was formed of a total of 250 participants; 125 (54 males, 71 females) of which were newly diagnosed with prediabetes, aged over 18 and who had not received any prior treatment and 125 (52 males, 73 females) healthy volunteers. Prediabeticpatients were diagnosed using a glucose tolerance test. In both groups, native thiol-disulfide exchanges were examined using the automated measurement method newly developed by Erel and Neselioglu. RESULTS: When compared to the control group, the native thiol (p<0.001) and total thiol (p=0.008) levels, and the native thiol/total thiol (p=0.022) ratio was lower; while disulfide (p=0.001) level and, disulfide/native thiol (p=0.003) and disulfide/total thiol (p=0.022) ratios were higher in prediabeticpatients. A positive correlation was determined between disulfide and the fasting blood glucose levels (r=0.394, p=0.017) and glycolysed hemoglobin (HbA1c) (r=0.307, p=0.011). On the other hand, a negative correlation was determined between native thiol and fasting blood glucose levels (r=-0.335, p=0.004). CONCLUSION: With this study, we have shown for the first time that thiol oxidation increases in prediabeticpatients and that there is a positive correlation between the disulfide and blood glucose and HbA1c levels.
Authors: Sevinc Can Sandikci; Seda Colak; Ahmet Omma; Mehmet E Enecik; Zeynep Ozbalkan; Salim Neselioglu; Ozcan Erel Journal: Arch Med Sci Date: 2019-07-18 Impact factor: 3.318