AIM: To test the efficacy and toxicity of exosomal doxorubicin (exoDOX) compared with free doxorubicin. MATERIALS & METHODS: The cytotoxic effects of exoDOX were tested in vitro and in nude mice by measuring the tumor volume. The toxic effects were evaluated by measuring the bodyweight and through histopathologic analyses. The biodistribution of DOX was assessed by MS. RESULTS: In vitro and in vivo studies showed that exosomes did not decrease the efficacy of DOX. Surprisingly, exoDOX showed no cardiotoxicity as observed in DOX-treated mice and MS studies confirmed that the accumulation of exoDOX in the heart was reduced by approximately 40%. CONCLUSION: We demonstrated that exoDOX was less toxic than DOX through its altered biodistribution.
AIM: To test the efficacy and toxicity of exosomal doxorubicin (exoDOX) compared with free doxorubicin. MATERIALS & METHODS: The cytotoxic effects of exoDOX were tested in vitro and in nude mice by measuring the tumor volume. The toxic effects were evaluated by measuring the bodyweight and through histopathologic analyses. The biodistribution of DOX was assessed by MS. RESULTS: In vitro and in vivo studies showed that exosomes did not decrease the efficacy of DOX. Surprisingly, exoDOX showed no cardiotoxicity as observed in DOX-treated mice and MS studies confirmed that the accumulation of exoDOX in the heart was reduced by approximately 40%. CONCLUSION: We demonstrated that exoDOX was less toxic than DOX through its altered biodistribution.
Entities:
Keywords:
breast cancer; drug delivery; exosomal doxorubicin; exosome; nanomedicine; toxicity
Authors: Dionysios C Watson; Defne Bayik; Avinash Srivatsan; Cristina Bergamaschi; Antonio Valentin; Gang Niu; Jenifer Bear; Mitchell Monninger; Mei Sun; Aizea Morales-Kastresana; Jennifer C Jones; Barbara K Felber; Xiaoyuan Chen; Ihsan Gursel; George N Pavlakis Journal: Biomaterials Date: 2016-07-06 Impact factor: 12.479