Literature DB >> 26416864

The Pathogen-Derived Aminoglycoside Resistance 16S rRNA Methyltransferase NpmA Possesses Dual m1A1408/m1G1408 Specificity.

Natalia Zelinskaya1, Marta A Witek1, Graeme L Conn2.   

Abstract

Chemical modification of 16S rRNA can confer exceptionally high-level resistance to a diverse set of aminoglycoside antibiotics. Here, we show that the pathogen-derived enzyme NpmA possesses dual m(1)A1408/m(1)G1408 activity, an unexpected property apparently unique among the known aminoglycoside resistance 16S rRNA (m(1)A1408) methyltransferases. Although the biological significance of this activity remains to be determined, such mechanistic variation in enzymes acquired by pathogens has significant implications for development of inhibitors of these emerging resistance determinants.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.

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Year:  2015        PMID： 26416864      PMCID： PMC4649141          DOI： 10.1128/AAC.01872-15

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  21 in total

1.  Expression, purification and crystallization of adenosine 1408 aminoglycoside-resistance rRNA methyltransferases for structural studies.

Authors:  Natalia Zelinskaya; C Robert Rankin; Rachel Macmaster; Miloje Savic; Graeme L Conn
Journal:  Protein Expr Purif       Date:  2010-07-25       Impact factor: 1.650

2.  Novel plasmid-mediated 16S rRNA m1A1408 methyltransferase, NpmA, found in a clinically isolated Escherichia coli strain resistant to structurally diverse aminoglycosides.

Authors:  Jun-ichi Wachino; Keigo Shibayama; Hiroshi Kurokawa; Kouji Kimura; Kunikazu Yamane; Satowa Suzuki; Naohiro Shibata; Yasuyoshi Ike; Yoshichika Arakawa
Journal:  Antimicrob Agents Chemother       Date:  2007-09-17       Impact factor: 5.191

3.  Structural basis for the methylation of G1405 in 16S rRNA by aminoglycoside resistance methyltransferase Sgm from an antibiotic producer: a diversity of active sites in m7G methyltransferases.

Authors:  Nilofer Husain; Karolina L Tkaczuk; Shenoy Rajesh Tulsidas; Katarzyna H Kaminska; Sonja Cubrilo; Gordana Maravić-Vlahovicek; Janusz M Bujnicki; J Sivaraman
Journal:  Nucleic Acids Res       Date:  2010-03-01       Impact factor: 16.971

4.  Structural insights into the function of aminoglycoside-resistance A1408 16S rRNA methyltransferases from antibiotic-producing and human pathogenic bacteria.

Authors:  Rachel Macmaster; Natalia Zelinskaya; Miloje Savic; C Robert Rankin; Graeme L Conn
Journal:  Nucleic Acids Res       Date:  2010-07-17       Impact factor: 16.971

5.  New archaeal methyltransferases forming 1-methyladenosine or 1-methyladenosine and 1-methylguanosine at position 9 of tRNA.

Authors:  Morgane Kempenaers; Martine Roovers; Yamina Oudjama; Karolina L Tkaczuk; Janusz M Bujnicki; Louis Droogmans
Journal:  Nucleic Acids Res       Date:  2010-06-04       Impact factor: 16.971

6.  Identification of the biosynthetic gene cluster and an additional gene for resistance to the antituberculosis drug capreomycin.

Authors:  Elizabeth A Felnagle; Michelle R Rondon; Andrew D Berti; Heidi A Crosby; Michael G Thomas
Journal:  Appl Environ Microbiol       Date:  2007-05-11       Impact factor: 4.792

7.  The aminoglycoside resistance methyltransferase Sgm impedes RsmF methylation at an adjacent rRNA nucleotide in the ribosomal A site.

Authors:  Sonja Cubrilo; Fedora Babić; Stephen Douthwaite; Gordana Maravić Vlahovicek
Journal:  RNA       Date:  2009-06-09       Impact factor: 4.942

8.  Structural bases for 16 S rRNA methylation catalyzed by ArmA and RmtB methyltransferases.

Authors:  Emmanuelle Schmitt; Marc Galimand; Michel Panvert; Patrice Courvalin; Yves Mechulam
Journal:  J Mol Biol       Date:  2009-03-20       Impact factor: 5.469

9.  Structural basis for the methylation of A1408 in 16S rRNA by a panaminoglycoside resistance methyltransferase NpmA from a clinical isolate and analysis of the NpmA interactions with the 30S ribosomal subunit.

Authors:  Nilofer Husain; Sonja Obranic; Lukasz Koscinski; J Seetharaman; Fedora Babic; Janusz M Bujnicki; Gordana Maravic-Vlahovicek; J Sivaraman
Journal:  Nucleic Acids Res       Date:  2010-11-09       Impact factor: 16.971

10.  Determination of the target nucleosides for members of two families of 16S rRNA methyltransferases that confer resistance to partially overlapping groups of aminoglycoside antibiotics.

Authors:  Miloje Savic; Josip Lovric; Tatjana Ilic Tomic; Branka Vasiljevic; Graeme L Conn
Journal:  Nucleic Acids Res       Date:  2009-07-09       Impact factor: 16.971
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  3 in total

1.  Substrate Recognition and Modification by a Pathogen-Associated Aminoglycoside Resistance 16S rRNA Methyltransferase.

Authors:  Kellie Vinal; Graeme L Conn
Journal:  Antimicrob Agents Chemother       Date:  2017-04-24       Impact factor: 5.191

2.  Structural and biochemical analysis of the dual-specificity Trm10 enzyme from Thermococcus kodakaraensis prompts reconsideration of its catalytic mechanism.

Authors:  Ranjan Kumar Singh; André Feller; Martine Roovers; Dany Van Elder; Lina Wauters; Louis Droogmans; Wim Versées
Journal:  RNA       Date:  2018-05-30       Impact factor: 4.942

3.  50S subunit recognition and modification by the Mycobacterium tuberculosis ribosomal RNA methyltransferase TlyA.

Authors:  Zane T Laughlin; Suparno Nandi; Debayan Dey; Natalia Zelinskaya; Marta A Witek; Pooja Srinivas; Ha An Nguyen; Emily G Kuiper; Lindsay R Comstock; Christine M Dunham; Graeme L Conn
Journal:  Proc Natl Acad Sci U S A       Date:  2022-03-31       Impact factor: 12.779
  3 in total

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