Qibin Qi1,2, Yan Zheng2, Tao Huang2, Jennifer Rood3, George A Bray3, Frank M Sacks2, Lu Qi4,5,6. 1. Department of Epidemiology and Population Health, Albert Einstein College of Medicine, Bronx, NY, USA. 2. Department of Nutrition, Harvard T.H. Chan School of Public Health, 665 Huntington Avenue, Boston, MA, 02115, USA. 3. LSU, Pennington Biomedical Research Center, Baton Rouge, LA, USA. 4. Department of Nutrition, Harvard T.H. Chan School of Public Health, 665 Huntington Avenue, Boston, MA, 02115, USA. lqi1@tulane.edu. 5. Department of Epidemiology, School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA, 70112, USA. lqi1@tulane.edu. 6. Channing Laboratory, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA. lqi1@tulane.edu.
Abstract
AIMS/HYPOTHESIS: Vitamin D and related genetic variants are associated with obesity and insulin resistance. We aimed to examine whether vitamin D metabolism-related variants affect changes in body weight and insulin resistance in response to weight-loss diets varying in macronutrient content. METHODS: Three vitamin D metabolism-related variants, DHCR7 rs12785878, CYP2R1 rs10741657 and GC rs2282679, were genotyped in 732 overweight/obese participants from a 2 year weight-loss trial (POUNDS Lost). We assessed genotype effects on changes in body weight, fasting levels of glucose and insulin, and HOMA-IR at 6 months (up to 656 participants) and 2 years (up to 596 participants) in response to low-protein vs high-protein diets, and low-fat vs high-fat diets. RESULTS: We found significant interactions between DHCR7 rs12785878 and diets varying in protein, but not in fat, on changes in insulin and HOMA-IR at both 6 months (p for interaction <0.001) and 2 years (p for interaction ≤ 0.03). The T allele (vitamin-D-increasing allele) of DHCR7 rs12785878 was associated with greater decreases in insulin and HOMA-IR (p < 0.002) in response to high-protein diets, while there was no significant genotype effect on changes in these traits in the low-protein diet group. Generalised estimating equation analyses indicated significant genotype effects on trajectory of changes in insulin resistance over the 2 year intervention in response to high-protein diets (p < 0.001). We did not observe significant interaction between the other two variants and dietary protein or fat on changes in these traits. CONCLUSIONS/ INTERPRETATION: Our data suggest that individuals carrying the T allele of DHCR7 rs12785878 might benefit more in improvement of insulin resistance than noncarriers by consuming high-protein weight-loss diets. TRIAL REGISTRATION: ClinicalTrials.gov NCT00072995.
RCT Entities:
AIMS/HYPOTHESIS: Vitamin D and related genetic variants are associated with obesity and insulin resistance. We aimed to examine whether vitamin D metabolism-related variants affect changes in body weight and insulin resistance in response to weight-loss diets varying in macronutrient content. METHODS: Three vitamin D metabolism-related variants, DHCR7rs12785878, CYP2R1rs10741657 and GC rs2282679, were genotyped in 732 overweight/obeseparticipants from a 2 year weight-loss trial (POUNDS Lost). We assessed genotype effects on changes in body weight, fasting levels of glucose and insulin, and HOMA-IR at 6 months (up to 656 participants) and 2 years (up to 596 participants) in response to low-protein vs high-protein diets, and low-fat vs high-fat diets. RESULTS: We found significant interactions between DHCR7rs12785878 and diets varying in protein, but not in fat, on changes in insulin and HOMA-IR at both 6 months (p for interaction <0.001) and 2 years (p for interaction ≤ 0.03). The T allele (vitamin-D-increasing allele) of DHCR7rs12785878 was associated with greater decreases in insulin and HOMA-IR (p < 0.002) in response to high-protein diets, while there was no significant genotype effect on changes in these traits in the low-protein diet group. Generalised estimating equation analyses indicated significant genotype effects on trajectory of changes in insulin resistance over the 2 year intervention in response to high-protein diets (p < 0.001). We did not observe significant interaction between the other two variants and dietary protein or fat on changes in these traits. CONCLUSIONS/ INTERPRETATION: Our data suggest that individuals carrying the T allele of DHCR7rs12785878 might benefit more in improvement of insulin resistance than noncarriers by consuming high-protein weight-loss diets. TRIAL REGISTRATION: ClinicalTrials.gov NCT00072995.
Authors: L Wamberg; T Christiansen; S K Paulsen; S Fisker; P Rask; L Rejnmark; B Richelsen; S B Pedersen Journal: Int J Obes (Lond) Date: 2012-07-17 Impact factor: 5.095
Authors: Rona J Strawbridge; Anna Deleskog; Olga McLeod; Lasse Folkersen; Maryam Kavousi; Karl Gertow; Damiano Baldassarre; Fabrizio Veglia; Karin Leander; Bruna Gigante; Jussi Kauhanen; Rainer Rauramaa; Andries J Smit; Elmo Mannarino; Philippe Giral; Abbas Dehghan; Albert Hofman; Oscar H Franco; Steve E Humphries; Elena Tremoli; Ulf de Faire; Sven Gustafsson; Claes-Göran Östensson; Per Eriksson; John Öhrvik; Anders Hamsten Journal: Diabetologia Date: 2014-03-25 Impact factor: 10.122
Authors: Ling Lu; Zhijie Yu; An Pan; Frank B Hu; Oscar H Franco; Huaixing Li; Xiaoying Li; Xilin Yang; Yan Chen; Xu Lin Journal: Diabetes Care Date: 2009-04-14 Impact factor: 17.152
Authors: Yiqing Song; Lu Wang; Anastassios G Pittas; Liana C Del Gobbo; Cuilin Zhang; Joann E Manson; Frank B Hu Journal: Diabetes Care Date: 2013-05 Impact factor: 19.112
Authors: Ángel M Martínez-Montes; Almudena Fernández; María Muñoz; Jose Luis Noguera; Josep M Folch; Ana I Fernández Journal: PLoS One Date: 2018-03-09 Impact factor: 3.240
Authors: Sridharan Raghavan; Kathleen Jablonski; Linda M Delahanty; Nisa M Maruthur; Aaron Leong; Paul W Franks; William C Knowler; Jose C Florez; Dana Dabelea Journal: Diabetes Obes Metab Date: 2021-01-29 Impact factor: 6.408