| Literature DB >> 26415755 |
Pan-Tao Zhang1, Chao Shan2, Xiao-Dan Li1, Si-Qing Liu2, Cheng-Lin Deng2, Han-Qing Ye2, Bao-Di Shang1, Pei-Yong Shi3, Ming Lv4, Bei-Fen Shen4, Cheng-Feng Qin5, Bo Zhang6.
Abstract
West Nile virus (WNV) is a neurotropic human pathogen that has caused increasing infected cases over recent years. There is currently no licensed vaccine or effective drug for prevention and treatment of WNV infection in humans. To facilitate antiviral drug discovery and neutralizing antibody detection, a WNV cDNA clone containing a luciferase reporter gene was constructed through incorporating Gaussia luciferase (Gluc) gene within the capsid-coding region of WNV genome. Transfection of BHK-21 cells with the cDNA clone-derived RNA generated luciferase reporter WNV (WNV-Gluc) and the stable WNV-Gluc with high titers (>10(7)PFU/ml) was obtained through plaque purification. Luciferase activity was used to effectively quantify the viral production of WNV-Gluc. Using the reporter virus WNV-Gluc, we developed a luciferase based assay in a 12-well format for evaluating neutralizing antibodies. The reporter virus could be a powerful tool for epidemiological investigation of WNV, vaccine evaluation, antiviral drug screening, and the study of WNV replication and pathogenesis.Entities:
Keywords: Antiviral drug discovery; Gaussia luciferase; Neutralizing antibody; Reporter virus; West Nile virus
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Year: 2015 PMID： 26415755 DOI： 10.1016/j.virusres.2015.09.015
Source DB: PubMed Journal: Virus Res ISSN： 0168-1702 Impact factor: 3.303