Literature DB >> 26408410

Recombinant K28 antigen in ELISA in the diagnosis of canine visceral leishmaniosis.

G L Venturin1, J P Bragato1, K L O Silva1, V M F de Lima2.   

Abstract

Crude total antigen (CTA) from Leishmania infantum and recombinant antigen K39 (rK39) and recombinant antigen K28 (rK28) were compared using an ELISA for the diagnosis of canine visceral leishmaniosis (CVL). Forty-two blood samples from healthy dogs from a nonendemic area and 80 blood samples from an endemic area for dogs with visceral leishmaniosis (VL), confirmed with positive parasitological tests for Leishmania spp., were used in an ELISA. The parasitological diagnosis was chosen as a gold standard. The ELISA with rK28 antigen showed sensitivity of 100% and specificity of 100%, high agreement with CTA and rK39, indicating that the rK28 antigen is useful for ELISA serological diagnosis of CVL.
© 2015 John Wiley & Sons Ltd.

Entities:  

Keywords:  ELISA; Leishmania infantum; parasitic; serology; zoonoses

Mesh：

Substances：

Year:  2015        PMID： 26408410     DOI： 10.1111/pim.12281

Source DB:  PubMed          Journal:  Parasite Immunol        ISSN： 0141-9838            Impact factor:   2.280


  6 in total

Review 1.  Canine visceral leishmaniasis: Diagnosis and management of the reservoir living among us.

Authors:  Bruno L Travi; Anabela Cordeiro-da-Silva; Filipe Dantas-Torres; Guadalupe Miró
Journal:  PLoS Negl Trop Dis       Date:  2018-01-11

2.  Data on differentially expressed miRNAs in dogs infected with Leishmania infantum.

Authors:  Jaqueline Poleto Bragato; Larissa Martins Melo; Gabriela Lovizutto Venturin; Gabriela Torres Rebech; Leandro Encarnação Garcia; Flavia Lombardi Lopes; Valéria Marçal Felix de Lima
Journal:  Data Brief       Date:  2018-01-09

3.  The Use of Specific Serological Biomarkers to Detect CaniLeish Vaccination in Dogs.

Authors:  Carla Lima; Nuno Santarém; Javier Nieto; Javier Moreno; Eugenia Carrillo; Daniella Castanheira Bartholomeu; Lilian Lacerda Bueno; Ricardo Fujiwara; Célia Amorim; Anabela Cordeiro-da-Silva
Journal:  Front Vet Sci       Date:  2019-10-24

4.  Revisiting the heterogeneous global genomic population structure of Leishmania infantum.

Authors:  Luz H Patino; Adriana Castillo-Castañeda; Marina Muñoz; Carlos Muskus; Matilde Rivero-Rodríguez; Alveiro Pérez-Doria; Eduar E Bejarano; Juan David Ramírez
Journal:  Microb Genom       Date:  2021-09

Review 5.  Developments in Diagnosis of Visceral Leishmaniasis in the Elimination Era.

Authors:  Om Prakash Singh; Shyam Sundar
Journal:  J Parasitol Res       Date:  2015-12-30

6.  Leishmania infantum recombinant kinesin degenerated derived repeat (rKDDR): A novel potential antigen for serodiagnosis of visceral leishmaniasis.

Authors:  Lucas Dhom-Lemos; Agostinho Gonçalves Viana; João Luis Reis Cunha; Mariana Santos Cardoso; Tiago Antônio Oliveira Mendes; Guilherme Rafael Gomide Pinheiro; Williane Fernanda Siqueira; Francisco Pereira Lobo; Leandro Freitas Teles; Lilian Lacerda Bueno; Silvio Fernando Guimarães-Carvalho; Daniella Castanheira Bartholomeu; Ricardo Toshio Fujiwara
Journal:  PLoS One       Date:  2019-01-31       Impact factor: 3.240
  6 in total

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