Literature DB >> 26407257

Comparison of Whole-Genome Sequencing and Molecular-Epidemiological Techniques for Clostridium difficile Strain Typing.

Samuel R Dominguez1, Lydia J Anderson2, Cassandra V Kotter3, Cynthia A Littlehorn4, Lesley E Arms4, Elaine Dowell4, James K Todd1, Daniel N Frank3.   

Abstract

We analyzed in parallel 27 pediatric Clostridium difficile isolates by repetitive sequence-based polymerase chain reaction (RepPCR), pulsed-field gel electrophoresis (PFGE), and whole-genome next-generation sequencing. Next-generation sequencing distinguished 3 groups of isolates that were indistinguishable by RepPCR and 1 isolate that clustered in the same PFGE group as other isolates.
© The Author 2015. Published by Oxford University Press on behalf of the Pediatric Infectious Diseases Society. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

Entities:  

Keywords:  Clostridium difficile; next generation sequencing; outbreak investigation; pediatrics

Mesh：

Year:  2015        PMID： 26407257     DOI： 10.1093/jpids/piv020

Source DB:  PubMed          Journal:  J Pediatric Infect Dis Soc        ISSN： 2048-7193            Impact factor:   3.164


  6 in total

1.  Investigation of Respiratory Syncytial Virus Outbreak on an Adult Stem Cell Transplant Unit by Use of Whole-Genome Sequencing.

Authors:  Yijun Zhu; Teresa R Zembower; Kristen E Metzger; Zhengdeng Lei; Stefan J Green; Chao Qi
Journal:  J Clin Microbiol       Date:  2017-07-26       Impact factor: 5.948

2.  Evaluation of bloodstream infections, Clostridium difficile infections, and gut microbiota in pediatric oncology patients.

Authors:  Bryan T Nycz; Samuel R Dominguez; Deborah Friedman; Joanne M Hilden; Diana Ir; Charles E Robertson; Daniel N Frank
Journal:  PLoS One       Date:  2018-01-12       Impact factor: 3.240

Review 3.  Over a Decade of recA and tly Gene Sequence Typing of the Skin Bacterium Propionibacterium acnes: What Have We Learnt?

Authors:  Andrew McDowell
Journal:  Microorganisms       Date:  2017-12-21

4.  Whole genome sequencing of Clostridioides difficile PCR ribotype 046 suggests transmission between pigs and humans.

Authors:  Anders Werner; Paula Mölling; Anna Fagerström; Fredrik Dyrkell; Dimitrios Arnellos; Karin Johansson; Martin Sundqvist; Torbjörn Norén
Journal:  PLoS One       Date:  2020-12-21       Impact factor: 3.240

Review 5.  Advances in the Microbiome: Applications to Clostridium difficile Infection.

Authors:  Eamonn P Culligan; Roy D Sleator
Journal:  J Clin Med       Date:  2016-09-21       Impact factor: 4.241

6.  Genomic evolution of Staphylococcus aureus isolates colonizing the nares and progressing to bacteremia.

Authors:  Jeanne B Benoit; Daniel N Frank; Mary T Bessesen
Journal:  PLoS One       Date:  2018-05-03       Impact factor: 3.240
  6 in total

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