Tao Wang1, Feng Ji2, Zhitang Dai2, Yue Xie2, Dongtang Yuan2. 1. Department of Orthopedics, the Affiliated Hospital of Qinghai University, Xining, Qinghai, China. 2. Department of Orthopedics, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu, China.
Abstract
BACKGROUND: MicroRNA (miR)-191 has been observed to be overexpressed in osteosarcoma cell lines in comparison with osteoblasts. OBJECTIVE: To investigate the clinical significance of miR-191 in human osteosarcomas. METHODS: Quantitative PCR was performed to detect miR-191 expression in osteosarcoma tissues and patients' sera. RESULTS: miR-191 expression levels, both in osteosarcoma tissues and patients' sera, were significantly higher than those in matched adjacent normal bone tissues and healthy controls (both P< 0.001). Importantly, miR-191 could efficiently screen osteosarcoma patients from healthy controls (Area under receiver operating characteristic curve, AUC = 0.808). Then, high serum miR-191 expression was significantly associated with advanced clinical stage (P = 0.001), large tumor size (P = 0.01) and positive distant metastasis (P = 0.001). Moreover, overall and disease-free survival durations in patients with high miR-191 expression were both shorter than those with low miR-191 expression. Multivariate analysis further identified serum miR-191 level as an independent and significant prognostic factor for both overall survival (P = 0.01) and disease-free survival (P = 0.02). CONCLUSIONS: Our data provide new insights for the involvement of miR-191 in osteosarcoma and suggest that the increased expression of miR-191 may be associated with aggressive tumor progression and adverse outcome. Of note, serum miR-191 quantification may be a promising biomarker for the diagnosis and prognosis in osteosarcoma.
BACKGROUND:MicroRNA (miR)-191 has been observed to be overexpressed in osteosarcoma cell lines in comparison with osteoblasts. OBJECTIVE: To investigate the clinical significance of miR-191 in humanosteosarcomas. METHODS: Quantitative PCR was performed to detect miR-191 expression in osteosarcoma tissues and patients' sera. RESULTS:miR-191 expression levels, both in osteosarcoma tissues and patients' sera, were significantly higher than those in matched adjacent normal bone tissues and healthy controls (both P< 0.001). Importantly, miR-191 could efficiently screen osteosarcomapatients from healthy controls (Area under receiver operating characteristic curve, AUC = 0.808). Then, high serum miR-191 expression was significantly associated with advanced clinical stage (P = 0.001), large tumor size (P = 0.01) and positive distant metastasis (P = 0.001). Moreover, overall and disease-free survival durations in patients with high miR-191 expression were both shorter than those with low miR-191 expression. Multivariate analysis further identified serum miR-191 level as an independent and significant prognostic factor for both overall survival (P = 0.01) and disease-free survival (P = 0.02). CONCLUSIONS: Our data provide new insights for the involvement of miR-191 in osteosarcoma and suggest that the increased expression of miR-191 may be associated with aggressive tumor progression and adverse outcome. Of note, serum miR-191 quantification may be a promising biomarker for the diagnosis and prognosis in osteosarcoma.