Yongqing Li1, Yadong Xu2, Chundong Yu3, Wenshu Zuo1. 1. Breast Cancer Center, Shandong Cancer Hospital, Jinan, Shandong, China. 2. Department of the Second General Surgery, People's Hospital of Haiyang, Yantai, Shandong, China. 3. Department of Laboratory, Women and Children's Hospital of Qingdao, Qingdao, Shandong, China.
Abstract
BACKGROUND: MicroRNAs (miRNA) expression profiles might be useful novel biomarkers for tumor diagnostic and histological characterization. OBJECTIVE: Associations of miR-146 expression and breast cancer in very young women needed to be elucidated. METHODS: We investigated expressions of miR-146a, miR-146b and IL-6 in tissues between 120 young women with primary breast cancer and 130 patients with breast fibroadenoma by RT-PCR. The associations between the expression of miR-146, IL-6 and clinical parameters of breast cancer were analyzed. RESULTS: Levels of miR-146a and miR-146b in breast cancer tissues were lower while level of IL-6 was higher compared to the fibroadenoma tissues and pericancerous breast tissues (P< 0.05). Positive associations were found between levels of miR-146a/b and IL-6 in breast cancer tissues and ER-PgR-, HER2/neu-, Ki-67 index ≥ 20%, tumor size > 2 cm, positive distant metastasis and lymph node metastasis, advanced clinical TNM stages (III-IV) and basal-like phenotype (P< 0.05). CONCLUSION: Down-regulations of miR-146a and miR-146b expression in breast tissues were related to development and deterioration of breast cancer. miR-146a and miR-146b might act as potential biomarkers for young women with breast cancer.
BACKGROUND: MicroRNAs (miRNA) expression profiles might be useful novel biomarkers for tumor diagnostic and histological characterization. OBJECTIVE: Associations of miR-146 expression and breast cancer in very young women needed to be elucidated. METHODS: We investigated expressions of miR-146a, miR-146b and IL-6 in tissues between 120 young women with primary breast cancer and 130 patients with breast fibroadenoma by RT-PCR. The associations between the expression of miR-146, IL-6 and clinical parameters of breast cancer were analyzed. RESULTS: Levels of miR-146a and miR-146b in breast cancer tissues were lower while level of IL-6 was higher compared to the fibroadenoma tissues and pericancerous breast tissues (P< 0.05). Positive associations were found between levels of miR-146a/b and IL-6 in breast cancer tissues and ER-PgR-, HER2/neu-, Ki-67 index ≥ 20%, tumor size > 2 cm, positive distant metastasis and lymph node metastasis, advanced clinical TNM stages (III-IV) and basal-like phenotype (P< 0.05). CONCLUSION: Down-regulations of miR-146a and miR-146b expression in breast tissues were related to development and deterioration of breast cancer. miR-146a and miR-146b might act as potential biomarkers for young women with breast cancer.
Entities:
Keywords:
Breast cancer; RT-PCR; miR-146a; miR-146b
Authors: Debashree Basudhar; Gaurav Bharadwaj; Veena Somasundaram; Robert Y S Cheng; Lisa A Ridnour; Mayumi Fujita; Stephen J Lockett; Stephen K Anderson; Daniel W McVicar; David A Wink Journal: Br J Pharmacol Date: 2018-11-06 Impact factor: 8.739