Nooshin Ayremlou1, Hossein Mozdarani1, Seyed Javad Mowla2, Alireza Delavari3. 1. Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. 2. Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. 3. Digestive Disease Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Abstract
BACKGROUND: MicroRNAs can function as oncogenes and tumor suppressor genes in human cancers. The expression of miR-107 is high in various types of solid tumors. OBJECTIVE: The aim of this study was to evaluate the tumor and serum level of miR-107 and its correlation to HIF-1α expression in gastric cancer patients. METHODS: Quantitative real-time PCR was used to analyze the expression of miR-107 and HIF-1α in 36 pairs of fresh gastric cancer and matched adjacent normal tissue specimens and the serum of these patients compared to age matched controls. RESULTS: The expression level of miR-107 was significantly higher in tumor tissues compared to adjacent normal tissue (p= 0.04). For serum, the expression level of miR-107 was significantly higher in gastric cancer patients than in age matched controls (p= 0.04). The correlation between tumor and serum expression of miR-107 with tumor hypoxia was found to be significant (p≤ 0.001). CONCLUSION: The overexpression of miR-107 in tumors and serum of gastric cancer patients and its correlation with HIF-1α expression in tumor tissues was indicated that miR-107 may have a potential to use as a biomarker for detection of gastric cancer patients and hypoxia in gastric cancer tumor.
BACKGROUND: MicroRNAs can function as oncogenes and tumor suppressor genes in humancancers. The expression of miR-107 is high in various types of solid tumors. OBJECTIVE: The aim of this study was to evaluate the tumor and serum level of miR-107 and its correlation to HIF-1α expression in gastric cancerpatients. METHODS: Quantitative real-time PCR was used to analyze the expression of miR-107 and HIF-1α in 36 pairs of fresh gastric cancer and matched adjacent normal tissue specimens and the serum of these patients compared to age matched controls. RESULTS: The expression level of miR-107 was significantly higher in tumor tissues compared to adjacent normal tissue (p= 0.04). For serum, the expression level of miR-107 was significantly higher in gastric cancerpatients than in age matched controls (p= 0.04). The correlation between tumor and serum expression of miR-107 with tumor hypoxia was found to be significant (p≤ 0.001). CONCLUSION: The overexpression of miR-107 in tumors and serum of gastric cancerpatients and its correlation with HIF-1α expression in tumor tissues was indicated that miR-107 may have a potential to use as a biomarker for detection of gastric cancerpatients and hypoxia in gastric cancer tumor.
Authors: María Teresa Corcuera; Cruz Rodríguez-Bobada; Jaime Zuloaga; Fernando Gómez-Aguado; Rosa Rodríguez-Perez; Ángel Mendizabal; Pablo González; Javier Arias-Díaz; María Luisa Caballero Journal: Parasitol Res Date: 2018-07-13 Impact factor: 2.289
Authors: Amelie Beaumier; Sally R Robinson; Nicholas Robinson; Katherine E Lopez; Dawn M Meola; Lisa G Barber; Barret J Bulmer; Jerome Calvalido; John E Rush; Ashish Yeri; Saumya Das; Vicky K Yang Journal: J Vet Intern Med Date: 2020-04-07 Impact factor: 3.333