Literature DB >> 26406327

Correction: Molecular Cloning, Heterologous Expression, and Functional Characterization of an NADPH-Cytochrome P450 Reductase Gene from Camptotheca acuminata, a Camptothecin-Producing Plant.

Xixing Qu, Xiang Pu, Fei Chen, Yun Yang, Lixia Yang, Guolin Zhang, Yinggang Luo.   

Abstract

Entities:  

Year:  2015        PMID: 26406327      PMCID: PMC4583399          DOI: 10.1371/journal.pone.0139647

Source DB:  PubMed          Journal:  PLoS One        ISSN: 1932-6203            Impact factor:   3.240


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The images for Figs 7 and 8 are incorrectly switched. The image that appears as Fig 7 should be Fig 8, and the image that appears as Fig 8 should be Fig 7. The figure captions appear in the correct order. Please see the corrected Fig 7 here.
Fig 7

Amino acid residues alignment of CamCPR, caa_locus_6894, caa_locus_12198, and caa_locus_112450 using Clustal Omega multiple aligment tool.

The identical amino acid residues between CamCPR and caa_locus_6894 were highlighted in red, between CamCPR and caa_locus_12198 were in green, and between CamCPR and caa_locus_112450 were in blue.

Fig 8

Enzymatic digestion and chemical degradation of tCamCPR.

The predicted cleavage sites of tCamCPR (a) and the detected peptide fragments by SDS-PAGE analysis (b). T, thrombin; H, Hydroxylamine; M, protein ruler; Lane 1, tCamCPR; Lane 2, thrombin; Enzyme digestion products of tCamCPR with 1 U / 50 μL (Lane 3) and 2 U / 50 μL (Lane 4) of thrombin were used [37]; Lanes 5 and 6, chemical degradation products of tCamCPR by hydroxylamine [38].

Amino acid residues alignment of CamCPR, caa_locus_6894, caa_locus_12198, and caa_locus_112450 using Clustal Omega multiple aligment tool.

The identical amino acid residues between CamCPR and caa_locus_6894 were highlighted in red, between CamCPR and caa_locus_12198 were in green, and between CamCPR and caa_locus_112450 were in blue. Please see the corrected Fig 8 here.

Enzymatic digestion and chemical degradation of tCamCPR.

The predicted cleavage sites of tCamCPR (a) and the detected peptide fragments by SDS-PAGE analysis (b). T, thrombin; H, Hydroxylamine; M, protein ruler; Lane 1, tCamCPR; Lane 2, thrombin; Enzyme digestion products of tCamCPR with 1 U / 50 μL (Lane 3) and 2 U / 50 μL (Lane 4) of thrombin were used [37]; Lanes 5 and 6, chemical degradation products of tCamCPR by hydroxylamine [38].
  1 in total

1.  Molecular Cloning, Heterologous Expression, and Functional Characterization of an NADPH-Cytochrome P450 Reductase Gene from Camptotheca acuminata, a Camptothecin-Producing Plant.

Authors:  Xixing Qu; Xiang Pu; Fei Chen; Yun Yang; Lixia Yang; Guolin Zhang; Yinggang Luo
Journal:  PLoS One       Date:  2015-08-07       Impact factor: 3.240

  1 in total

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