Matthew T Houdek1, Cody C Wyles2, Benjamin D Packard3, Andre Terzic4, Atta Behfar4, Rafael J Sierra1. 1. Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota; Center for Regenerative Medicine, Mayo Clinic, Rochester, Minnesota. 2. Mayo Medical School, Mayo Clinic, Rochester, Minnesota; Center for Regenerative Medicine, Mayo Clinic, Rochester, Minnesota. 3. Center for Regenerative Medicine, Mayo Clinic, Rochester, Minnesota. 4. Division of Cardiovascular Diseases, Mayo Clinic, Rochester, Minnesota; Center for Regenerative Medicine, Mayo Clinic, Rochester, Minnesota.
Abstract
BACKGROUND: Osteonecrosis (ON) of the femoral head occurs when cells of trabecular bone spontaneously die. Mesenchymal stem cells (MSCs) have been introduced into the femoral head in an attempt to halt progression of the disease. The purpose of this study was to functionally compare MSCs in patients with ON of the femoral head with patients without. METHODS: Mesenchymal stem cells were isolated from 20 patients with corticosteroid-induced ON and 10 controls without. Colony-forming unit and proliferation assays were used to assess MSC proliferation. Mesenchymal stem cells were differentiated into bone, fat, and cartilage. Functional assays were used to quantify the differentiation capacity. RESULTS: Control MSCs demonstrated greater cellular growth potential and improved ability to differentiate into bone. CONCLUSION: The decreased ability to differentiate into bone may be a reason why patients treated with autologous MSC infusion fail regenerative treatment strategies and progress to collapse.
BACKGROUND:Osteonecrosis (ON) of the femoral head occurs when cells of trabecular bone spontaneously die. Mesenchymal stem cells (MSCs) have been introduced into the femoral head in an attempt to halt progression of the disease. The purpose of this study was to functionally compare MSCs in patients with ON of the femoral head with patients without. METHODS: Mesenchymal stem cells were isolated from 20 patients with corticosteroid-induced ON and 10 controls without. Colony-forming unit and proliferation assays were used to assess MSC proliferation. Mesenchymal stem cells were differentiated into bone, fat, and cartilage. Functional assays were used to quantify the differentiation capacity. RESULTS: Control MSCs demonstrated greater cellular growth potential and improved ability to differentiate into bone. CONCLUSION: The decreased ability to differentiate into bone may be a reason why patients treated with autologous MSC infusion fail regenerative treatment strategies and progress to collapse.
Authors: Cody C Wyles; Christopher R Paradise; Matthew T Houdek; Susan L Slager; Andre Terzic; Atta Behfar; Andre J van Wijnen; Rafael J Sierra Journal: Clin Orthop Relat Res Date: 2019-08 Impact factor: 4.176
Authors: Zheng Li; Chao Jiang; Xingye Li; William K K Wu; Xi Chen; Shibai Zhu; Chanhua Ye; Matthew T V Chan; Wenwei Qian Journal: Cell Prolif Date: 2017-12-04 Impact factor: 6.831
Authors: Matthew T Houdek; Cody C Wyles; Mark S Collins; Benjamin M Howe; Andre Terzic; Atta Behfar; Rafael J Sierra Journal: Clin Orthop Relat Res Date: 2018-02 Impact factor: 4.176