| Literature DB >> 26403847 |
Yahya E Jad1, Gerardo A Acosta2,3, Sherine N Khattab4, Beatriz G de la Torre1, Thavendran Govender1, Hendrik G Kruger1, Ayman El-Faham5,6, Fernando Albericio7,8,9,10,11,12.
Abstract
2-MeTHF and CPME were evaluated as greener alternatives for the most employed solvents in peptide synthesis. The ability of these solvents to dissolve amino acid derivatives and a range of coupling reagents were evaluated as well as the swelling of polystyrene and polyethylene glycol resins. In addition, racemization and coupling efficiencies were also determined. We concluded that the use of 2-MeTHF with combination of DIC/OxymaPure gave the lowest racemization level during stepwise synthesis of Z-Phg-Pro-NH2 and the highest purity during SPPS of Aib-enkephalin pentapeptide (H-Tyr-Aib-Aib-Phe-Leu-NH2).Entities:
Keywords: 2-methyltetrahydrofuran; Cyclopentyl methyl ether; Green solvents; Peptide synthesis; Solid-phase peptide synthesis
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Year: 2015 PMID： 26403847 DOI： 10.1007/s00726-015-2095-x
Source DB: PubMed Journal: Amino Acids ISSN： 0939-4451 Impact factor: 3.520