BACKGROUND: To evaluate the incidence and the risk of transmitting a virus through a bone allograft from a living donor. MATERIAL AND METHODS: A total 7032 femoral heads have been collected from 24 Belgian institutions. The tissue along with the screening blood tests were systematically sent to the bank. Serological screening included: for HIV, a HIV1-2 antibody test; for HBV, a HBS antigen and HBS and Hbcore antibodies; for HCV, a HCV antibody test. Syphilis was also screened with a non-specific and a specific assays. HTLV1-2 screening was recommended but not obligatory. RESULTS: From the 7032 femoral heads, 1066 (15.2%) implants were definitively excluded. Hundred forty-six femoral heads, representing 2.1% of all grafts and 13.9% of the excluded ones, were discarded for positive serological testing associated with a risk of disease transmission. There were 2 donors who tested positive for HTLV1-2. The prevalence of HIV in the femoral head donor population was six times lower than in the general one. The prevalence of hepatitis B and C was similar but far higher than HIV. The risk was computed to be 0.54 out of 1 × 10(5) for HIV and HCV without quarantine or tissue processing. For HBV, the risk was 0.77 out of 1 × 10(5). CONCLUSION: Current standards of tissue banking incorporated safety and quality as their main features. This policy is now regulated at the European level. With a multi-step screening-policy, stringent donor selection guidelines, the risk of viral transmission trough a tissue is minimized.
BACKGROUND: To evaluate the incidence and the risk of transmitting a virus through a bone allograft from a living donor. MATERIAL AND METHODS: A total 7032 femoral heads have been collected from 24 Belgian institutions. The tissue along with the screening blood tests were systematically sent to the bank. Serological screening included: for HIV, a HIV1-2 antibody test; for HBV, a HBS antigen and HBS and Hbcore antibodies; for HCV, a HCV antibody test. Syphilis was also screened with a non-specific and a specific assays. HTLV1-2 screening was recommended but not obligatory. RESULTS: From the 7032 femoral heads, 1066 (15.2%) implants were definitively excluded. Hundred forty-six femoral heads, representing 2.1% of all grafts and 13.9% of the excluded ones, were discarded for positive serological testing associated with a risk of disease transmission. There were 2 donors who tested positive for HTLV1-2. The prevalence of HIV in the femoral head donor population was six times lower than in the general one. The prevalence of hepatitis B and C was similar but far higher than HIV. The risk was computed to be 0.54 out of 1 × 10(5) for HIV and HCV without quarantine or tissue processing. For HBV, the risk was 0.77 out of 1 × 10(5). CONCLUSION: Current standards of tissue banking incorporated safety and quality as their main features. This policy is now regulated at the European level. With a multi-step screening-policy, stringent donor selection guidelines, the risk of viral transmission trough a tissue is minimized.
Authors: Leslie H Tobler; Susan L Stramer; Stephen R Lee; Barbara L Masecar; Jon E Peterson; E Anne Davis; William E Andrews; Jaye P Brodsky; Steven H Kleinman; Bruce H Phelps; Michael P Busch Journal: Transfusion Date: 2003-10 Impact factor: 3.157
Authors: C R Horsburgh; C Y Ou; J Jason; S D Holmberg; I M Longini; C Schable; K H Mayer; A R Lifson; G Schochetman; J W Ward Journal: Lancet Date: 1989-09-16 Impact factor: 79.321
Authors: Barna D Tugwell; Priti R Patel; Ian T Williams; Katrina Hedberg; Feng Chai; Omana V Nainan; Ann R Thomas; Judith E Woll; Beth P Bell; Paul R Cieslak Journal: Ann Intern Med Date: 2005-11-01 Impact factor: 25.391
Authors: R J Simonds; S D Holmberg; R L Hurwitz; T R Coleman; S Bottenfield; L J Conley; S H Kohlenberg; K G Castro; B A Dahan; C A Schable Journal: N Engl J Med Date: 1992-03-12 Impact factor: 91.245