Wirginia Krzyściak1, Anna Jurczak2, Jakub Piątkowski3, Dorota Kościelniak2, Iwona Gregorczyk-Maga2, Iwona Kołodziej2, Monika A Papież4, Dorota Olczak-Kowalczyk5. 1. Department of Medical Diagnostics, Faculty of Pharmacy, Jagiellonian University Medical College, Krakow, Poland. 2. Department of Pediatric Dentistry, Institute of Dentistry, Jagiellonian University Medical College, Krakow, Poland. 3. Genetics Laboratory, Department of Endocrinology, Jagiellonian University Medical College, Krakow, Poland. 4. Department of Cytobiology, Faculty of Pharmacy, Jagiellonian University Medical College, Krakow, Poland. 5. Department of Pediatric Dentistry, Medical University of Warsaw, Warsaw, Poland.
Abstract
INTRODUCTION: The mechanisms of adhesion to solid surfaces enable S. mutans to colonize oral cavities and form biofilms, which play an important role in caries development. Additional properties enabling the survival of S. mutans in the oral cavity include its ability to survive in acidic environments and specific interactions with other microorganisms inhabiting this ecosystem. AIM OF THE STUDY: The aim of this study was to determine the antibacterial activity of saliva histatin-5 (peptide) and lysozyme (protein) against S. mutans and L. rhamnosus, as representatives of physiological flora. MATERIALS AND METHODS: The study involved strains of physiological (L. rhamnosus) and cariogenic (S. mutans) flora isolated from one patient with diagnosed early caries of the deciduous teeth. RESULTS: It was proved that the presence of probiotic L. rhamnosus bacteria in the environment had a negative impact on the ability of S. mutans to produce biofilm. Moreover, the antibacterial activity of histatin-5 was confirmed, and it inhibited S. mutans growth at concentrations of 27.2 μg/ml and 54.4 μg/ml, both individually and in a mixture with lysozyme (in a total concentration of 54.4 μg/ml). CONCLUSIONS: The data obtained constitute a promising result due to their potential future application in the prevention and early diagnosis of caries.
INTRODUCTION: The mechanisms of adhesion to solid surfaces enable S. mutans to colonize oral cavities and form biofilms, which play an important role in caries development. Additional properties enabling the survival of S. mutans in the oral cavity include its ability to survive in acidic environments and specific interactions with other microorganisms inhabiting this ecosystem. AIM OF THE STUDY: The aim of this study was to determine the antibacterial activity of saliva histatin-5 (peptide) and lysozyme (protein) against S. mutans and L. rhamnosus, as representatives of physiological flora. MATERIALS AND METHODS: The study involved strains of physiological (L. rhamnosus) and cariogenic (S. mutans) flora isolated from one patient with diagnosed early caries of the deciduous teeth. RESULTS: It was proved that the presence of probiotic L. rhamnosus bacteria in the environment had a negative impact on the ability of S. mutans to produce biofilm. Moreover, the antibacterial activity of histatin-5 was confirmed, and it inhibited S. mutans growth at concentrations of 27.2 μg/ml and 54.4 μg/ml, both individually and in a mixture with lysozyme (in a total concentration of 54.4 μg/ml). CONCLUSIONS: The data obtained constitute a promising result due to their potential future application in the prevention and early diagnosis of caries.
Authors: Blanca Blancas; María de Lourdes Lanzagorta; Luis Felipe Jiménez-Garcia; Reyna Lara; José Luis Molinari; Ana María Fernández Journal: Clin Exp Dent Res Date: 2021-05-05
Authors: Galina Laputková; Vladimíra Schwartzová; Juraj Bánovčin; Michal Alexovič; Ján Sabo Journal: Open Life Sci Date: 2018-05-18 Impact factor: 0.938