Patricia G Couto1, Luciana Bastos-Rodrigues2, Juliana G Carneiro3, Fernanda Guieiro1, Maria Aparecida Bicalho4, Franciele B Leidenz1, Ana J Bicalho1, Eitan Friedman5, Luiz De Marco6. 1. Department of Surgery, Universidade Federal de Minas Gerais, Av. Alfredo Balena 190, Room 114, Belo Horizonte, 30130-100, Brazil. 2. Universidade Federal de Juiz de Fora, Campus Governador Valadares, Governador Valadares, Minas Gerais, Brazil. 3. Faculty of Medical Science, Centro de Ensino Superior e Desenvolvimento, Campina Grande, Brazil. 4. Department of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. 5. The Susanne Levy Gertner Oncogenetics Unit, Chaim Sheba Medical Center, Tel-Hashomer, Israel. 6. Department of Surgery, Universidade Federal de Minas Gerais, Av. Alfredo Balena 190, Room 114, Belo Horizonte, 30130-100, Brazil. Ldemarco@ufmg.br.
Abstract
INTRODUCTION: Lung cancer is the leading global cause of cancer-related mortality and is associated with poor prognosis. To improve survival rates of lung cancer patients, better understanding of tumorigenic mechanisms is necessary, which may lead to development of new therapeutic strategies. The hOGG1 and NTH1 genes act in the DNA BER repair pathway and their involvement in lung cancer pathogenesis has been analyzed in several populations. METHODS: We analyzed targeted regions of the hOGG1 and NTH1 genes in 96 Brazilian patients with non-small-cell lung cancer (NSCLC) and 89 cancer-free, ethnically matched controls. RESULTS: The NTH1 c.98G>T polymorphism rs2302172 (p = 0.02 and p = 0.02 for allele and genotype frequency between cases and controls, respectively) and the 140-17C> T variant (rs2233518) (p = 0.02 and p = 0.02 for allele and genotype frequency between cases and controls, respectively) were detected in four lung cancer cases (4 %) while the NTH1 Q131K (C391A) polymorphism was found in seven lung cancer cases (7 %) (p = 0.001 and p = 0.008, for allele and genotype frequency between cases and controls, respectively). None of these sequence variants were detected in controls. The Ser326Cys (C1245G, rs1052133) polymorphism in the OGG1 gene was detected in 42 % of analyzed NSCLC patients and in 34 % of the controls (p = 0.11 and p = 0.25 for allele and genotype frequency between cases and controls, respectively). CONCLUSIONS: Our study provides preliminary evidence that polymorphisms in OGG1 do not contribute to development of NSCLC in Brazilian patients and that NTH1 polymorphisms may be associated with NSCLC pathogenesis.
INTRODUCTION:Lung cancer is the leading global cause of cancer-related mortality and is associated with poor prognosis. To improve survival rates of lung cancerpatients, better understanding of tumorigenic mechanisms is necessary, which may lead to development of new therapeutic strategies. The hOGG1 and NTH1 genes act in the DNA BER repair pathway and their involvement in lung cancer pathogenesis has been analyzed in several populations. METHODS: We analyzed targeted regions of the hOGG1 and NTH1 genes in 96 Brazilian patients with non-small-cell lung cancer (NSCLC) and 89 cancer-free, ethnically matched controls. RESULTS: The NTH1 c.98G>T polymorphism rs2302172 (p = 0.02 and p = 0.02 for allele and genotype frequency between cases and controls, respectively) and the 140-17C> T variant (rs2233518) (p = 0.02 and p = 0.02 for allele and genotype frequency between cases and controls, respectively) were detected in four lung cancer cases (4 %) while the NTH1Q131K (C391A) polymorphism was found in seven lung cancer cases (7 %) (p = 0.001 and p = 0.008, for allele and genotype frequency between cases and controls, respectively). None of these sequence variants were detected in controls. The Ser326Cys (C1245G, rs1052133) polymorphism in the OGG1 gene was detected in 42 % of analyzed NSCLCpatients and in 34 % of the controls (p = 0.11 and p = 0.25 for allele and genotype frequency between cases and controls, respectively). CONCLUSIONS: Our study provides preliminary evidence that polymorphisms in OGG1 do not contribute to development of NSCLC in Brazilian patients and that NTH1 polymorphisms may be associated with NSCLC pathogenesis.
Authors: Jeffrey S Chang; Margaret R Wrensch; Helen M Hansen; Jennette D Sison; Melinda C Aldrich; Charles P Quesenberry; Michael F Seldin; Karl T Kelsey; John K Wiencke Journal: Carcinogenesis Date: 2008-11-24 Impact factor: 4.944
Authors: Aaron C Jacobs; Marcus J Calkins; Ajit Jadhav; Dorjbal Dorjsuren; David Maloney; Anton Simeonov; Pawel Jaruga; Miral Dizdaroglu; Amanda K McCullough; R Stephen Lloyd Journal: PLoS One Date: 2013-12-09 Impact factor: 3.240