L Yang1, H-S Bai, Y Deng, L Fan. 1. Department of Oncology, Sichuan Provincial People's Hospital, School of Clinical Medicine of University of Electronic Science and Technology of China, Chengdu, China. linfani1@126.com.
Abstract
OBJECTIVE: Although the oncogenic role of long non-coding RNA, MALAT1 in cervical cancer is gradually recognized, the clinical and prognostic significance of this lncRNA in cervical cancer has not been reported yet. This study aimed to investigate the clinical significance and biological functions of MALAT1 in cervical cancer. PATIENTS AND METHODS: MALAT1 expression in 104 cervical cancer tissues and matched adjacent normal tissues, as well as in 50 HPV negative healthy cervical tissues were quantified using qRT-PCR. Its association with overall survival of the cancer patients was analyzed using the Log-rank (Mantel-Cox) test and the Cox proportional hazards model. In addition, the effect of MALAT1 on cell proliferation and invasion was further studied in Hela and CaSki cells. RESULTS: MALAT1 expression is significantly increased in cervical cancer than in normal tissues. Its expression in the cancerous tissues is also significantly higher than in adjacent normal tissues. MALAT1 expression is correlated with tumor size, FIGO stage, vascular invasion and lymph nodes metastasis and is an independent predictor for overall survival of cervical cancer. When endogenous MALAT1 was knocked down, the cancer cells had significantly reduced proliferation and invasion and increased apoptosis. CONCLUSIONS: MALAT1 might be an important marker of prognosis and a potential therapeutic target of cervical cancer.
OBJECTIVE: Although the oncogenic role of long non-coding RNA, MALAT1 in cervical cancer is gradually recognized, the clinical and prognostic significance of this lncRNA in cervical cancer has not been reported yet. This study aimed to investigate the clinical significance and biological functions of MALAT1 in cervical cancer. PATIENTS AND METHODS: MALAT1 expression in 104 cervical cancer tissues and matched adjacent normal tissues, as well as in 50 HPV negative healthy cervical tissues were quantified using qRT-PCR. Its association with overall survival of the cancerpatients was analyzed using the Log-rank (Mantel-Cox) test and the Cox proportional hazards model. In addition, the effect of MALAT1 on cell proliferation and invasion was further studied in Hela and CaSki cells. RESULTS:MALAT1 expression is significantly increased in cervical cancer than in normal tissues. Its expression in the cancerous tissues is also significantly higher than in adjacent normal tissues. MALAT1 expression is correlated with tumor size, FIGO stage, vascular invasion and lymph nodes metastasis and is an independent predictor for overall survival of cervical cancer. When endogenous MALAT1 was knocked down, the cancer cells had significantly reduced proliferation and invasion and increased apoptosis. CONCLUSIONS:MALAT1 might be an important marker of prognosis and a potential therapeutic target of cervical cancer.