Rei Kawashima1,2, Mio Fujimaki3, Yuka Ikenoue3, Keiko Danjo3, Wasaburo Koizumi4, Takafumi Ichikawa5. 1. Department of Regulation Biochemistry, Kitasato University Graduate School of Medical Sciences, 1-15-1 Kitasato, Minami-ku, Sagamahara, Kanagawa, 252-0373, Japan. 2. Department of Gastroenterology, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku-ku, Tokyo, 162-8655, Japan. 3. Reseach Institute, Pharmaceutical Research Laboratories, Ajinomoto Co., Inc., Suzuki-cho, Kawasaki-ku, Kawasaki, Kanagawa, 210-8681, Japan. 4. Department of Gastroenterology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamahara, Kanagawa, 252-0373, Japan. 5. Department of Regulation Biochemistry, Kitasato University Graduate School of Medical Sciences, 1-15-1 Kitasato, Minami-ku, Sagamahara, Kanagawa, 252-0373, Japan. t.ichika@kitasato-u.ac.jp.
Abstract
PURPOSE: The elemental diet (ED) Elental® reportedly reduces adverse reactions to chemotherapy in digestive system cancer patients; however, the mechanism is unclear. Therefore, we verified the protective effect of ED against gastrointestinal disorders induced by the antineoplastic drug 5-fluorouracil (5-FU). METHODS: After 5 days of tail vein injections of 40 mg/kg/day 5-FU in female BALB/c mice, the mice were given oral ED (ED group) or dextrin with the same number of calories (control group). We measured the weight of salivary glands and the PAS-positive area of colonic mucosa and verified the antitumor effect in tumor-bearing mice given 5-FU and ED. RESULTS: Although body weight decreased after 5-FU treatment, ED group mice weighed more than control group mice. Additionally, although control mice developed diarrhea after 5-FU treatment, the ED group showed only loose stools. The control group saliva volume was approximately one sixth of the vehicle group volume after 5-FU treatment; this was improved to approximately half in the ED group. The area ratio of PAS-positive cells in the colonic mucosa was reduced by 5-FU treatment, with the ratio being higher in the ED group than that in the control group. Similar tumor growth suppression was observed in the 5-FU and ED groups. CONCLUSIONS: ED alleviated adverse reactions to 5-FU without affecting antitumor activity. Protection against 5-FU-induced weight loss was potentially due to both improved nutritional support with combined ingredients and prevention of diarrhea that is associated with reduced colonic goblet cells and decreased saliva production from reduced salivary gland contraction.
PURPOSE: The elemental diet (ED) Elental® reportedly reduces adverse reactions to chemotherapy in digestive system cancerpatients; however, the mechanism is unclear. Therefore, we verified the protective effect of ED against gastrointestinal disorders induced by the antineoplastic drug 5-fluorouracil (5-FU). METHODS: After 5 days of tail vein injections of 40 mg/kg/day 5-FU in female BALB/c mice, the mice were given oral ED (ED group) or dextrin with the same number of calories (control group). We measured the weight of salivary glands and the PAS-positive area of colonic mucosa and verified the antitumor effect in tumor-bearing mice given 5-FU and ED. RESULTS: Although body weight decreased after 5-FU treatment, ED group mice weighed more than control group mice. Additionally, although control mice developed diarrhea after 5-FU treatment, the ED group showed only loose stools. The control group saliva volume was approximately one sixth of the vehicle group volume after 5-FU treatment; this was improved to approximately half in the ED group. The area ratio of PAS-positive cells in the colonic mucosa was reduced by 5-FU treatment, with the ratio being higher in the ED group than that in the control group. Similar tumor growth suppression was observed in the 5-FU and ED groups. CONCLUSIONS: ED alleviated adverse reactions to 5-FU without affecting antitumor activity. Protection against 5-FU-induced weight loss was potentially due to both improved nutritional support with combined ingredients and prevention of diarrhea that is associated with reduced colonic goblet cells and decreased saliva production from reduced salivary gland contraction.
Authors: Linda S Elting; Catherine Cooksley; Mark Chambers; Scott B Cantor; Ellen Manzullo; Edward B Rubenstein Journal: Cancer Date: 2003-10-01 Impact factor: 6.860
Authors: Lázaro de Sousa Fideles; João Antônio Leal de Miranda; Conceição da Silva Martins; Maria Lucianny Lima Barbosa; Helder Bindá Pimenta; Paulo Vitor de Souza Pimentel; Claudio Silva Teixeira; Marina Alves Sampaio Scafuri; Samuel de Osterno Façanha; João Erivan Façanha Barreto; Poliana Moreira de Medeiros Carvalho; Ariel Gustavo Scafuri; Joabe Lima Araújo; Jefferson Almeida Rocha; Icaro Gusmão Pinto Vieira; Nágila Maria Pontes Silva Ricardo; Matheus da Silva Campelo; Maria Elenir Nobre Pinho Ribeiro; Gerly Anne de Castro Brito; Gilberto Santos Cerqueira Journal: Molecules Date: 2020-06-17 Impact factor: 4.411