A M Pabst1, C Walter1, A Bell2, M Weyhrauch3, I Schmidtmann4, H Scheller3, K M Lehmann5. 1. Department of Oral- and Maxillofacial Surgery, University Medical Center, Augustusplatz 2, 55131, Mainz, Germany. 2. Department of Oral Surgery, University Medical Center, Augustusplatz 2, 55131, Mainz, Germany. 3. Department of Prosthodontics, University Medical Center, Augustusplatz 2, 55131, Mainz, Germany. 4. Institute of Medical Biostatistics, Epidemiology and Informatics (IMBEI), University Medical Center, Obere Zahlbacher Str. 69, 55131, Mainz, Germany. 5. Department of Prosthodontics, University Medical Center, Augustusplatz 2, 55131, Mainz, Germany. drkarllehmann@gmail.com.
Abstract
OBJECTIVES: This study investigated the influence of three CAD/CAM zirconia ceramics for implant-abutment manufacturing on cell viability, migration ability, and cytotoxicity of human gingival fibroblasts (HGF) and oral keratinocytes (HOK) in vitro. MATERIALS AND METHODS: HGF and HOK were cultured on zirconia ceramic disks (VITA In-Ceram YZ, Ivoclar IPS e.max ZirCAD, Sirona inCoris ZI) and on control disks made of tissue culture polystyrene. Cell viability was analyzed by a MTT assay. Migration ability was detected by a scratch assay. A ToxiLight assay was used to analyze the influence of the tested zirconia ceramics on adenylate kinase (ADK) release and cytotoxicity. RESULTS: At MTT assay, HGF showed an increased cell viability compared to the control after 9 and 12 days for all ceramics (p each ≤0.0002) while HOK demonstrated a decreased cell viability after 9 and 12 days for all ceramics (p each ≤0.0003). At scratch assay, HGF exhibited for all ceramics decreased relative distances of the scratch wound compared to the control from 24 to 48 h (p each <0.0001) with exception of VITA In-Ceram YZ after 48 h. HOK showed increased distances compared to the control for all ceramics after 48 h (p each <0.0001). At ToxiLight assay, a minimal cytotoxicity of the tested materials could be detected. CONCLUSIONS: Overall, significant influences of the investigated CAD/CAM zirconia ceramics on HGF and HOK could be shown. CLINICAL RELEVANCE: The analyzed zirconia ceramics could influence oral soft-tissue cells that might affect the esthetic outcome after implant placement using CAD/CAM zirconia abutments.
OBJECTIVES: This study investigated the influence of three CAD/CAMzirconia ceramics for implant-abutment manufacturing on cell viability, migration ability, and cytotoxicity of human gingival fibroblasts (HGF) and oral keratinocytes (HOK) in vitro. MATERIALS AND METHODS:HGF and HOK were cultured on zirconia ceramic disks (VITA In-Ceram YZ, Ivoclar IPS e.max ZirCAD, Sirona inCoris ZI) and on control disks made of tissue culture polystyrene. Cell viability was analyzed by a MTT assay. Migration ability was detected by a scratch assay. A ToxiLight assay was used to analyze the influence of the tested zirconia ceramics on adenylate kinase (ADK) release and cytotoxicity. RESULTS: At MTT assay, HGF showed an increased cell viability compared to the control after 9 and 12 days for all ceramics (p each ≤0.0002) while HOK demonstrated a decreased cell viability after 9 and 12 days for all ceramics (p each ≤0.0003). At scratch assay, HGF exhibited for all ceramics decreased relative distances of the scratch wound compared to the control from 24 to 48 h (p each <0.0001) with exception of VITA In-Ceram YZ after 48 h. HOK showed increased distances compared to the control for all ceramics after 48 h (p each <0.0001). At ToxiLight assay, a minimal cytotoxicity of the tested materials could be detected. CONCLUSIONS: Overall, significant influences of the investigated CAD/CAMzirconia ceramics on HGF and HOK could be shown. CLINICAL RELEVANCE: The analyzed zirconia ceramics could influence oral soft-tissue cells that might affect the esthetic outcome after implant placement using CAD/CAMzirconia abutments.
Authors: Thomas Ziebart; Anne Schnell; Christian Walter; Peer W Kämmerer; Andreas Pabst; Karl M Lehmann; Johanna Ziebart; Marc O Klein; Bilal Al-Nawas Journal: Clin Oral Investig Date: 2012-03-10 Impact factor: 3.573
Authors: Dan Dominik Brüllmann; Andreas Pabst; Karl M Lehmann; Thomas Ziebart; Marc O Klein; Bernd d'Hoedt Journal: Clin Oral Investig Date: 2010-10-15 Impact factor: 3.573
Authors: Irena Sailer; Aurel Fehér; Frank Filser; Ludwig J Gauckler; Heinz Lüthy; Christoph Hans Franz Hämmerle Journal: Int J Prosthodont Date: 2007 Jul-Aug Impact factor: 1.681
Authors: A M Pabst; W Wagner; A Kasaj; S Gebhardt; M Ackermann; A Astolfo; F Marone; D Haberthür; F Enzmann; Moritz A Konerding Journal: Clin Oral Investig Date: 2014-09-11 Impact factor: 3.573