Raghavendra Shrishail Medikeri1, Suresh Vasant Lele2, Pradnya Prabhakar Mali3, Pinal Mahendra Jain3, Dattatray Anant Darawade4, Manjushri Raghavendra Medikeri5. 1. Reader, Department of Periodontology, Sinhgad Dental College and Hospital , Off sinhgad Road, Pune, Maharashtra, India . 2. Professor and Head, Department of Periodontology, Sinhgad Dental College and Hospital , Off sinhgad Road, Pune, Maharashtra, India . 3. Research Student, Department of Periodontology, Sinhgad Dental College and Hospital , Off sinhgad Road, Pune, Maharashtra, India . 4. Professor, Department of Oral and Maxillofacial Surgery, Rangta College of Dental Sciences and Research , Bhilai, Chattisgarh, India . 5. Reader, Department of Oral Medicine and Radiology, Sinhgad Dental College and Hospital , Off sinhgad Road, Pune, Maharashtra, India .
Abstract
BACKGROUND AND AIM: Periodontopathogens require iron constituents for their growth and metabolism in subgingival crevice. In this study, C.rectus was detected and quantified by using 16s rDNA based PCR in chronic periodontitis and compared with the levels of serum iron, total iron binding capacity and transferrin in chronic periodontitis and healthy sites. MATERIALS AND METHODS: One hundred twenty subjects divided into chronic periodontitis and healthy controls. Deep subgingival plaque was collected and genomic DNA was extracted from each sample analysed for C.rectus using 16s rRNA based PCR analysis. Blood samples were collected from both groups for estimation of serum iron, serum total iron binding capacity and serum transferrin levels. The quantified bacterial count was compared with blood samples. C. rectus was detected in both groups. RESULTS: There was significant increase in bacterial count in chronic periodontitis (p<0.05). Serum iron level was significantly raised in healthy group. TIBC and transferrin levels were elevated in periodontitis. Although these differences were non-significant. Regression analysis showed significant linear relationship between C.rectus counts and decreasing iron levels and consequently increasing serum transferrin and TIBC (p<0.05). CONCLUSION: The preliminary in vivo findings suggests C.rectus requires iron as a significant source of nutrition for its survival and growth form its hosts in deeper subgingival sites.
BACKGROUND AND AIM: Periodontopathogens require iron constituents for their growth and metabolism in subgingival crevice. In this study, C.rectus was detected and quantified by using 16s rDNA based PCR in chronic periodontitis and compared with the levels of serum iron, total iron binding capacity and transferrin in chronic periodontitis and healthy sites. MATERIALS AND METHODS: One hundred twenty subjects divided into chronic periodontitis and healthy controls. Deep subgingival plaque was collected and genomic DNA was extracted from each sample analysed for C.rectus using 16s rRNA based PCR analysis. Blood samples were collected from both groups for estimation of serum iron, serum total iron binding capacity and serum transferrin levels. The quantified bacterial count was compared with blood samples. C. rectus was detected in both groups. RESULTS: There was significant increase in bacterial count in chronic periodontitis (p<0.05). Serum iron level was significantly raised in healthy group. TIBC and transferrin levels were elevated in periodontitis. Although these differences were non-significant. Regression analysis showed significant linear relationship between C.rectus counts and decreasing iron levels and consequently increasing serum transferrin and TIBC (p<0.05). CONCLUSION: The preliminary in vivo findings suggests C.rectus requires iron as a significant source of nutrition for its survival and growth form its hosts in deeper subgingival sites.
Authors: Jimmy Y W Lam; Alan K L Wu; Dickson C Ngai; Jade L L Teng; Elsa S Y Wong; Susanna K P Lau; Rodney A Lee; Patrick C Y Woo Journal: J Clin Microbiol Date: 2011-01-26 Impact factor: 5.948
Authors: F Dati; G Schumann; L Thomas; F Aguzzi; S Baudner; J Bienvenu; O Blaabjerg; S Blirup-Jensen; A Carlström; P H Petersen; A M Johnson; A Milford-Ward; R F Ritchie; P J Svendsen; J Whicher Journal: Eur J Clin Chem Clin Biochem Date: 1996-06