Literature DB >> 26392991

Is bleach-sedimented smear microscopy an alternative to direct microscopy under programme conditions in India?

P H Vishnu1, P Bhat2, A Bansal2, S Satyanarayana3, U Alavadi2, B S Ohri4, M S Rao Shrinivas5, P Desikan6, J Jaju2, V G Rao7, P K Moonan8.   

Abstract

This cross-sectional multi-centric study compared the yield of and potential benefit for detecting smear-positive pulmonary tuberculosis (PTB) by bleach sedimentation (2% sodium-hypochlorite) versus direct microscopy under programme conditions in India. Among 3168 PTB suspects, 684 (21.6%) were detected by bleach sedimentation vs. 625 (19.7%) by direct microscopy, with a proportional overall agreement of 96% (κ = 0.88). While 594 patients were smear-positive with both methods, 31 patients detected by direct microscopy were missed and an additional 90 patients were detected by bleach sedimentation. Overall, bleach sedimentation increased the yield of smear-positive TB detection; however; it also increased the time to results.

Entities:  

Keywords:  TB diagnosis; bleach sedimentation; bleach smears; conventional microscopy; direct smears

Year:  2013        PMID： 26392991      PMCID： PMC4463091          DOI： 10.5588/pha.12.0100

Source DB:  PubMed          Journal:  Public Health Action        ISSN： 2220-8372


  8 in total

1.  Light-emitting diode with various sputum smear preparation techniques to diagnose tuberculosis.

Authors:  M Habtamu; J van den Boogaard; A Ndaro; R Buretta; C F Irongo; D A Lega; B M Nyombi; G S Kibiki
Journal:  Int J Tuberc Lung Dis       Date:  2012       Impact factor: 2.373

2.  Clorox digestion; a comparison of clorox digestion and three other methods for finding acid-fast organisms in sputum.

Authors:  G M CAMERON; R CASTLES
Journal:  Am Rev Tuberc       Date:  1945-12

Review 3.  Sputum processing methods to improve the sensitivity of smear microscopy for tuberculosis: a systematic review.

Authors:  Karen R Steingart; Vivienne Ng; Megan Henry; Philip C Hopewell; Andrew Ramsay; Jane Cunningham; Richard Urbanczik; Mark D Perkins; Mohamed Abdel Aziz; Madhukar Pai
Journal:  Lancet Infect Dis       Date:  2006-10       Impact factor: 25.071

4.  Bleach sedimentation: an opportunity to optimize smear microscopy for tuberculosis diagnosis in settings of high prevalence of HIV.

Authors:  Maryline Bonnet; Andrew Ramsay; Willie Githui; Laramie Gagnidze; Francis Varaine; Philippe J Guerin
Journal:  Clin Infect Dis       Date:  2008-06-01       Impact factor: 9.079

5.  Improved sensitivity of direct microscopy for acid-fast bacilli: sedimentation as an alternative to centrifugation for concentration of tubercle bacilli.

Authors:  H Miörner; G Ganlöv; Z Yohannes; Y Adane
Journal:  J Clin Microbiol       Date:  1996-12       Impact factor: 5.948

6.  Present position of microscopy and of culture in diagnostic mycobacteriology.

Authors:  R Urbanczik
Journal:  Zentralbl Bakteriol Mikrobiol Hyg A       Date:  1985-08

7.  Transmission of Mycobacterium tuberculosis from patients smear-negative for acid-fast bacilli.

Authors:  M A Behr; S A Warren; H Salamon; P C Hopewell; A Ponce de Leon; C L Daley; P M Small
Journal:  Lancet       Date:  1999-02-06       Impact factor: 79.321

8.  Commercial nucleic-acid amplification tests for diagnosis of pulmonary tuberculosis in respiratory specimens: meta-analysis and meta-regression.

Authors:  Daphne I Ling; Laura L Flores; Lee W Riley; Madhukar Pai
Journal:  PLoS One       Date:  2008-02-06       Impact factor: 3.240
  8 in total
  3 in total

1.  Promoting operational research through fellowships: a case study from the South-East Asia Union Office.

Authors:  A M V Kumar; S Satyanarayana; S Dar Berger; S S Chadha; R J Singh; P Lal; J Tonsing; A D Harries
Journal:  Public Health Action       Date:  2015-03-21

2.  The Mono-Prep system increases the detection rate of sputum smear microscopy for diagnosing tuberculosis.

Authors:  Jiannan Wu; Chengcheng Kong; Fengmin Huo; Qian Liang; Yifeng Ma; Yuanyuan Shang; Liping Zhao; Jian Du; Zhaogang Sun
Journal:  J Int Med Res       Date:  2018-09-04       Impact factor: 1.671

3.  Operational research within a Global Fund supported tuberculosis project in India: why, how and its contribution towards change in policy and practice.

Authors:  Karuna D Sagili; Srinath Satyanarayana; Sarabjit S Chadha; Nevin C Wilson; Ajay M V Kumar; Patrick K Moonan; John E Oeltmann; Vineet K Chadha; Sharath Burugina Nagaraja; Smita Ghosh; Terrence Q Lo; Tyson Volkmann; Matthew Willis; Kalpita Shringarpure; Ravichandra Chinnappa Reddy; Prahlad Kumar; Sreenivas A Nair; Raghuram Rao; Mohammed Yassin; Perry Mwangala; Rony Zachariah; Jamhoih Tonsing; Anthony D Harries; Sunil Khaparde
Journal:  Glob Health Action       Date:  2018       Impact factor: 2.640
  3 in total

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