Manal Farea1, Adam Husein2, Ahmad Sukari Halim3, Zurairah Berahim2, Asma Abdullah Nurul4, Khairani Idah Mokhtar5, Kasmawati Mokhtar2. 1. Conservative Department, School of Dental Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia. ghorafi44@yahoo.com. 2. Conservative Department, School of Dental Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia. 3. Reconstructive Sciences Unit, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia. 4. School of Health Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia. 5. Department of Oral Biology, Kulliyah of Dentistry, IIUM Kuantan Campus, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200, Kuantan Pahang DM, Malaysia.
Abstract
OBJECTIVES: The purpose of this study was to evaluate the synergistic effect of epithelial rests of Malassez cells (ERM) and transforming growth factor-β1 (TGF-β1) on proliferation, cementogenic and osteogenic differentiation of stem cells derived from human exfoliated deciduous teeth (SHED). MATERIALS AND METHODS: SHED were co-cultured with ERM with/without TGF-β1. Then, SHED proliferation, morphological appearance, alkaline phosphatase (ALP) activity, mineralization behaviour and gene/protein expression of cemento/osteoblastic phenotype were evaluated. RESULTS: TGF-β1 enhanced SHED proliferation when either cultured alone or co-cultured with ERM. ERM induced the cementoblastic differentiation of SHED which was significantly accelerated when treated with TGF-β1. This activity was demonstrated by high ALP activity, strong mineral deposition and upregulation of cementum/bone-related gene and protein expressions (i.e. ALP, collagen type I, bone sialoprotein, osteocalcin and cementum attachment protein). CONCLUSIONS: ERM were able to induce SHED differentiation along the cemento/osteoblastic lineage that was triggered in the presence of TGF-β1. CLINICAL RELEVANCE: The cemento/osteoblastic differentiation capability of SHED possesses a therapeutic potential in endodontic and periodontal tissue engineering.
OBJECTIVES: The purpose of this study was to evaluate the synergistic effect of epithelial rests of Malassez cells (ERM) and transforming growth factor-β1 (TGF-β1) on proliferation, cementogenic and osteogenic differentiation of stem cells derived from human exfoliated deciduous teeth (SHED). MATERIALS AND METHODS: SHED were co-cultured with ERM with/without TGF-β1. Then, SHED proliferation, morphological appearance, alkaline phosphatase (ALP) activity, mineralization behaviour and gene/protein expression of cemento/osteoblastic phenotype were evaluated. RESULTS: TGF-β1 enhanced SHED proliferation when either cultured alone or co-cultured with ERM. ERM induced the cementoblastic differentiation of SHED which was significantly accelerated when treated with TGF-β1. This activity was demonstrated by high ALP activity, strong mineral deposition and upregulation of cementum/bone-related gene and protein expressions (i.e. ALP, collagen type I, bone sialoprotein, osteocalcin and cementum attachment protein). CONCLUSIONS: ERM were able to induce SHED differentiation along the cemento/osteoblastic lineage that was triggered in the presence of TGF-β1. CLINICAL RELEVANCE: The cemento/osteoblastic differentiation capability of SHED possesses a therapeutic potential in endodontic and periodontal tissue engineering.
Entities:
Keywords:
Cementogenic differentiation; Epithelial rests of Malassez cells; Osteogenic differentiation; Stem cells of human exfoliated deciduous teeth; Transforming growth factor-β1
Authors: Bernardo Cesar Costa; Guilherme José Pimentel Lopes de Oliveira; Maria das Graças Afonso Miranda Chaves; Renan Roberto da Costa; Mário Francisco Real Gabrielli; Juliane Maria Guerreiro-Tanomaru; Mário Tanomaru-Filho Journal: World J Clin Cases Date: 2016-09-16 Impact factor: 1.337