Literature DB >> 26392293

A novel protein extraction method for identification of mycobacteria using MALDI-ToF MS.

La'Tonzia L Adams1, Parichat Salee2, Kim Dionne3, Karen Carroll3, Nicole Parrish3.   

Abstract

Commercial extraction methods for identification of mycobacteria using MALDI-ToF MS are laborious and time consuming. We have developed a novel extraction method which utilizes a bead beater and zirconia/silica beads to significantly shorten the existing protocol. This novel method provides a more rapid extraction of mycobacteria versus the commercial standard.
Copyright © 2015 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  MALDI-ToF MS; Mycobacterium; Protein extraction

Mesh：

Year:  2015        PMID： 26392293     DOI： 10.1016/j.mimet.2015.09.010

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  9 in total

1.  Comparison of Saramis 4.12 and IVD 3.0 Vitek MS Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Mycobacteria from Solid and Liquid Culture Media.

Authors:  Caroline Leyer; Guillaume Gregorowicz; Faiza Mougari; Laurent Raskine; Emmanuelle Cambau; Dominique de Briel
Journal:  J Clin Microbiol       Date:  2017-04-19       Impact factor: 5.948

2.  Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Combined Species Identification and Drug Sensitivity Testing in Mycobacteria.

Authors:  Pieter-Jan Ceyssens; Karine Soetaert; Markus Timke; An Van den Bossche; Katrin Sparbier; Koen De Cremer; Markus Kostrzewa; Marijke Hendrickx; Vanessa Mathys
Journal:  J Clin Microbiol       Date:  2016-12-21       Impact factor: 5.948

3.  Biographical Feature: Karen C. Carroll, M.D.

Authors:  Erik Munson
Journal:  J Clin Microbiol       Date:  2021-07-19       Impact factor: 5.948

4.  Discrimination between regional biotypes of Impatiens glandulifera using a simple MALDI-TOF MS-based method for use with seeds.

Authors:  Michael A Reeve; Kathryn M Pollard
Journal:  Plant Methods       Date:  2019-03-14       Impact factor: 4.993

5.  Discrimination between Asian populations of the parasitoid wasp Ganaspis cf. brasiliensis using a simple MALDI-TOF MS-based method for use with insects.

Authors:  Michael A Reeve; M Lukas Seehausen
Journal:  Biol Methods Protoc       Date:  2019-03-18

6.  Identification of Penicillium species by MALDI-TOF MS analysis of spores collected by dielectrophoresis.

Authors:  Michael A Reeve; Denise Bachmann; Thelma S Caine
Journal:  Biol Methods Protoc       Date:  2019-11-29

7.  Biosafety and Proteome Profiles of Different Heat Inactivation Methods for Mycobacterium tuberculosis.

Authors:  Cheng-Hui Wang; Denise Utami Putri; Jau-Ching Lee; Chi-Chih Liao; Sung-Tzu Tsao; Ai-Lin Hsiao; Jhao-Hui Wu; Xiao-Wei Chen; Chih-Hsin Lee; I-Lin Tsai
Journal:  Microbiol Spectr       Date:  2021-12-22

8.  Differentiation between closely-related Impatiens spp. and regional biotypes of Impatiens glandulifera using a highly-simplified and inexpensive method for MALDI-TOF MS.

Authors:  Michael A Reeve; Kathryn M Pollard; Daisuke Kurose
Journal:  Plant Methods       Date:  2018-07-16       Impact factor: 4.993

9.  A simple and inexpensive method for practical storage of field-sample proteins for subsequent MALDI-TOF MS analysis.

Authors:  Michael A Reeve; Alan G Buddie
Journal:  Plant Methods       Date:  2018-10-15       Impact factor: 4.993
  9 in total

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