Cheng Z Wang1, Amina El Ayadi2, Juhi Goswamy3, Celeste C Finnerty4, Randy Mifflin2, Linda Sousse2, Perenlei Enkhbaatar5, John Papaconstantinou1, David N Herndon2, Naseem H Ansari6. 1. Department of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555-0647, United States. 2. Department of Surgery, University of Texas Medical Branch, Galveston, TX 77555-0647, United States; Shriners Hospital for Children, Galveston, TX, United States. 3. University of Miami Miller School of Medicine, Miami, FL 33124, United States. 4. Department of Surgery, University of Texas Medical Branch, Galveston, TX 77555-0647, United States; Institute for Translational Sciences, Galveston, TX, United States; Shriners Hospital for Children, Galveston, TX, United States. 5. Department of Anesthesiology, University of Texas Medical Branch, Galveston, TX 77555-0647, United States. 6. Department of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555-0647, United States. Electronic address: nansari@utmb.edu.
Abstract
UNLABELLED: Oxidative stress may be involved in the cellular damage and tissue destruction as burn wounds continues to progress after abatement of the initial insult. Since iron and calcium ions play key roles in oxidative stress, this study tested whether topical application of Livionex formulation (LF) lotion, that contains disodium EDTA as a metal chelator and methyl sulfonyl methane (MSM) as a permeability enhancer, would prevent or reduce burns. METHODS: We used an established brass comb burn model with some modifications. Topical application of LF lotion was started 5 min post-burn, and repeated every 8 h for 3 consecutive days. Rats were euthanized and skin harvested for histochemistry and immunohistochemistry. Formation of protein adducts of 4-hydroxynonenal (HNE), malonadialdehyde (MDA) and acrolein (ACR) and expression of aldehyde dehydrogenase (ALDH) isozymes, ALDH1 and ALDH2 were assessed. RESULTS: LF lotion-treated burn sites and interspaces showed mild morphological improvement compared to untreated burn sites. Furthermore, the lotion significantly decreased the immunostaining of lipid aldehyde-protein adducts including protein -HNE, -MDA and -ACR adducts, and restored the expression of aldehyde dehydrogenase isozymes in the unburned interspaces. CONCLUSION: This data, for the first time, demonstrates that a topically applied EDTA-containing lotion protects burns progression with a concomitant decrease in the accumulation of reactive lipid aldehydes and protection of aldehyde dehydrogenase isozymes. Present studies are suggestive of therapeutic intervention of burns by this novel lotion.
UNLABELLED: Oxidative stress may be involved in the cellular damage and tissue destruction as burn wounds continues to progress after abatement of the initial insult. Since iron and calcium ions play key roles in oxidative stress, this study tested whether topical application of Livionex formulation (LF) lotion, that contains disodium EDTA as a metal chelator and methyl sulfonyl methane (MSM) as a permeability enhancer, would prevent or reduce burns. METHODS: We used an established brass comb burn model with some modifications. Topical application of LF lotion was started 5 min post-burn, and repeated every 8 h for 3 consecutive days. Rats were euthanized and skin harvested for histochemistry and immunohistochemistry. Formation of protein adducts of 4-hydroxynonenal (HNE), malonadialdehyde (MDA) and acrolein (ACR) and expression of aldehyde dehydrogenase (ALDH) isozymes, ALDH1 and ALDH2 were assessed. RESULTS: LF lotion-treated burn sites and interspaces showed mild morphological improvement compared to untreated burn sites. Furthermore, the lotion significantly decreased the immunostaining of lipid aldehyde-protein adducts including protein -HNE, -MDA and -ACR adducts, and restored the expression of aldehyde dehydrogenase isozymes in the unburned interspaces. CONCLUSION: This data, for the first time, demonstrates that a topically applied EDTA-containing lotion protects burns progression with a concomitant decrease in the accumulation of reactive lipid aldehydes and protection of aldehyde dehydrogenase isozymes. Present studies are suggestive of therapeutic intervention of burns by this novel lotion.
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Authors: Amina El Ayadi; Cheng Z Wang; Min Zhang; Michael Wetzel; Anesh Prasai; Celeste C Finnerty; Perenlei Enkhbaatar; David N Herndon; Naseem H Ansari Journal: Burns Trauma Date: 2020-10-02
Authors: Amy L Bogue; Warunya Panmanee; Cameron T McDaniel; Joel E Mortensen; Edwin Kamau; Luis A Actis; Jay A Johannigman; Michael J Schurr; Latha Satish; Nalinikanth Kotagiri; Daniel J Hassett Journal: PLoS One Date: 2021-03-03 Impact factor: 3.240
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