Literature DB >> 26391199

Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-L-phenylalanine.

E H Abdelkader1, A Feintuch, X Yao, L A Adams, L Aurelio, B Graham, D Goldfarb, G Otting.   

Abstract

Quantitative cysteine-independent ligation of a Gd(3+) tag to genetically encoded p-azido-L-phenylalanine via Cu(I)-catalyzed click chemistry is shown to deliver an exceptionally powerful tool for Gd(3+)-Gd(3+) distance measurements by double electron-electron resonance (DEER) experiments, as the position of the Gd(3+) ion relative to the protein can be predicted with high accuracy.

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Year:  2015        PMID: 26391199     DOI: 10.1039/c5cc07121f

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN: 1359-7345            Impact factor:   6.222


  13 in total

1.  Synthesis and Explosion Hazards of 4-Azido-l-phenylalanine.

Authors:  Mark B Richardson; Derek B Brown; Carlos A Vasquez; Joseph W Ziller; Kevin M Johnston; Gregory A Weiss
Journal:  J Org Chem       Date:  2018-04-02       Impact factor: 4.354

2.  Pulse EPR-enabled interpretation of scarce pseudocontact shifts induced by lanthanide binding tags.

Authors:  Elwy H Abdelkader; Xuejun Yao; Akiva Feintuch; Luke A Adams; Luigi Aurelio; Bim Graham; Daniella Goldfarb; Gottfried Otting
Journal:  J Biomol NMR       Date:  2015-11-23       Impact factor: 2.835

3.  Conformational ensemble of a multidomain protein explored by Gd3+ electron paramagnetic resonance.

Authors:  Tomohide Saio; Soya Hiramatsu; Mizue Asada; Hiroshi Nakagawa; Kazumi Shimizu; Hiroyuki Kumeta; Toshikazu Nakamura; Koichiro Ishimori
Journal:  Biophys J       Date:  2021-07-07       Impact factor: 3.699

4.  Studying the Conformation of a Receptor Tyrosine Kinase in Solution by Inhibitor-Based Spin Labeling.

Authors:  Dongsheng M Yin; Jeffrey S Hannam; Anton Schmitz; Olav Schiemann; Gregor Hagelueken; Michael Famulok
Journal:  Angew Chem Int Ed Engl       Date:  2017-06-19       Impact factor: 15.336

5.  Double Nitroxide Labeling by Copper-Catalyzed Azide-Alkyne Cycloadditions with Noncanonical Amino Acids for Electron Paramagnetic Resonance Spectroscopy.

Authors:  Pia Widder; Frederic Berner; Daniel Summerer; Malte Drescher
Journal:  ACS Chem Biol       Date:  2019-05-01       Impact factor: 5.100

Review 6.  Expanding the Genetic Code for Site-Directed Spin-Labeling.

Authors:  Theresa Braun; Malte Drescher; Daniel Summerer
Journal:  Int J Mol Sci       Date:  2019-01-16       Impact factor: 5.923

7.  Protein Spin Labeling with a Photocaged Nitroxide Using Diels-Alder Chemistry.

Authors:  Anandi Kugele; Bjarne Silkenath; Jakob Langer; Valentin Wittmann; Malte Drescher
Journal:  Chembiochem       Date:  2019-08-14       Impact factor: 3.164

8.  Probing the solution structure of the E. coli multidrug transporter MdfA using DEER distance measurements with nitroxide and Gd(III) spin labels.

Authors:  Eliane H Yardeni; Thorsten Bahrenberg; Richard A Stein; Smriti Mishra; Elia Zomot; Bim Graham; Kellie L Tuck; Thomas Huber; Eitan Bibi; Hassane S Mchaourab; Daniella Goldfarb
Journal:  Sci Rep       Date:  2019-08-29       Impact factor: 4.379

9.  Aqueous synthesis of a small-molecule lanthanide chelator amenable to copper-free click chemistry.

Authors:  Stephanie C Bishop; Robert Winefield; Asokan Anbanandam; Jed N Lampe
Journal:  PLoS One       Date:  2019-03-27       Impact factor: 3.240

10.  In situ observation of conformational dynamics and protein ligand-substrate interactions in outer-membrane proteins with DEER/PELDOR spectroscopy.

Authors:  Benesh Joseph; Eva A Jaumann; Arthur Sikora; Katja Barth; Thomas F Prisner; David S Cafiso
Journal:  Nat Protoc       Date:  2019-07-05       Impact factor: 13.491

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