| Literature DB >> 26391061 |
Wang Jingbo1, Chen Aimin1, Wu Qi1, Li Xin1, Li Huaining2.
Abstract
Betulinic acid (BA), a triterpenoid isolated from birch bark, has been reported to have anti-inflammatory effects. In this study, we investigated the anti-osteoarthritic effects of BA in IL-1β-stimulated human osteoarthritis chondrocytes. Human osteoarthritis chondrocytes were pre-incubated with BA (6, 12, 24μM) for 12h and then treated with IL-1β (10ng/ml). The production of PGE2 and NO were detected by ELISA and Griess reagent. The expression of NF-κB, IκB, and PPAR-γ were detected by Western blotting. The results showed that BA dose-dependently inhibited IL-1β-induced MMP-1, MMP-3, MMP-13, PGE2 and NO productions. BA also inhibited IL-1β-induced NF-κB activation. Furthermore, BA was found to activate PPAR-γ and the inhibition of PGE2 and NO by BA can be reversed by PPAR-γ antagonist GW9662. In conclusion, these results suggested that BA inhibited IL-1β-induced inflammation in osteoarthritis chondrocytes by activating PPAR-γ.Entities:
Keywords: Betulinic acid; IL-1β; NF-κB; Osteoarthritis chondrocyte; PPAR-γ
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Year: 2015 PMID： 26391061 DOI： 10.1016/j.intimp.2015.09.009
Source DB: PubMed Journal: Int Immunopharmacol ISSN： 1567-5769 Impact factor: 4.932