Kwang Kyoun Kim1, Yougun Won2, Tae-Gyun Kim1, Myong-Hyun Baek3, Jaewon Choi4. 1. Department of Orthopedic Surgery, Konyang University College of Medicine, Daejeon, Korea. 2. Department of Orthopedic Surgery, Konyang University College of Medicine, Daejeon, Korea. ; Department of Orthopaedics, Graduate School of Medicine, Yonsei University, Seoul, Korea. 3. Medical Device Development Center, Medical Innovation Foundation, Osong, Korea. 4. Medicine Major, Konyang University College of Medicine, Daejeon, Korea.
Abstract
BACKGROUND: The purpose of this study is to investigate differences of chemical composition between subchondral bone in advanced osteoarthritic (OA) and non-OA distal femur. METHODS: Twenty femurs were harvested, respectively. The subchondral trabeculae were obtained from the middle of medial articular surface of distal femurs. A 10 mm diameter cylindrical saw was used to harvest. Raman spectroscopy, a non-destructive technique, was employed to determine the chemical information of the trabecular bones in the human distal femurs. RESULTS: The maximum intensity of the phosphate peak was 2,376.51±954.6 for the non-OA group and 1,936.3±831.75 for the OA group. The maximum intensity of the phosphate peak observed between the two groups was significantly different (P=0.017). The maximum intensity of the amide I peak were 474.17±253.42 for the nonOA group and 261.91±205.61 for the OA group. The maximum intensity of the amide I peak were significantly different between the two groups (P=0.042). Also, among other chemical and matrix components (Hydroxyproline,Carbonate, Amide IIIdisordered;ordered, and CH2), the spectrums showed similar significant differences in the intensity (P=0.027, P=0.014, P=0.012; P=0.038, P=0.029). Area integration were performed to determine disorder in collagen's secondary structure via amide III (alpha helix/random coil). The value of the alpha helix to random coil band area are significantly different (P=0.021) and result showing that there was a trend toward higher collagen maturity for the nonosteoarthritic bone specimens. CONCLUSIONS: The result suggested that OA may affect the chemical compositions of trabecular bone, and such distinctive chemical information may be.
BACKGROUND: The purpose of this study is to investigate differences of chemical composition between subchondral bone in advanced osteoarthritic (OA) and non-OA distal femur. METHODS: Twenty femurs were harvested, respectively. The subchondral trabeculae were obtained from the middle of medial articular surface of distal femurs. A 10 mm diameter cylindrical saw was used to harvest. Raman spectroscopy, a non-destructive technique, was employed to determine the chemical information of the trabecular bones in the human distal femurs. RESULTS: The maximum intensity of the phosphate peak was 2,376.51±954.6 for the non-OA group and 1,936.3±831.75 for the OA group. The maximum intensity of the phosphate peak observed between the two groups was significantly different (P=0.017). The maximum intensity of the amide I peak were 474.17±253.42 for the nonOA group and 261.91±205.61 for the OA group. The maximum intensity of the amide I peak were significantly different between the two groups (P=0.042). Also, among other chemical and matrix components (Hydroxyproline,Carbonate, Amide IIIdisordered;ordered, and CH2), the spectrums showed similar significant differences in the intensity (P=0.027, P=0.014, P=0.012; P=0.038, P=0.029). Area integration were performed to determine disorder in collagen's secondary structure via amide III (alpha helix/random coil). The value of the alpha helix to random coil band area are significantly different (P=0.021) and result showing that there was a trend toward higher collagen maturity for the nonosteoarthritic bone specimens. CONCLUSIONS: The result suggested that OA may affect the chemical compositions of trabecular bone, and such distinctive chemical information may be.
Authors: Karen A Dehring; Nicole J Crane; Abigail R Smukler; Jonathan B McHugh; Blake J Roessler; Michael D Morris Journal: Appl Spectrosc Date: 2006-10 Impact factor: 2.388
Authors: Tomasz Buchwald; Krzysztof Niciejewski; Marek Kozielski; Mirosław Szybowicz; Marcin Siatkowski; Hanna Krauss Journal: J Biomed Opt Date: 2012-01 Impact factor: 3.170
Authors: Grace Kim; Jacqueline H Cole; Adele L Boskey; Shefford P Baker; Marjolein C H van der Meulen Journal: Calcif Tissue Int Date: 2014-06-03 Impact factor: 4.333