Shinji Kitajima1, Yasunori Iwata2,3,4,5, Kengo Furuichi2,3,4,5, Akihiro Sagara2,3,4,5, Yasuyuki Shinozaki2,3,4,5, Tadashi Toyama2,3,4,5, Norihiko Sakai2,3,4,5, Miho Shimizu2,3,4,5, Takeshi Sakurai2,3,4,5, Shuichi Kaneko2,3,4,5, Takashi Wada2,3,4,5. 1. Division of Nephrology, Kanazawa University Hospital, 13-1 Takara-machi, Kanazawa, 920-8641, Japan. kitajimajima@ybb.ne.jp. 2. Division of Nephrology, Kanazawa University Hospital, 13-1 Takara-machi, Kanazawa, 920-8641, Japan. 3. Department of Molecular Neuroscience and Integrative Physiology, Faculty of Medicine, Kanazawa University, Kanazawa, Japan. 4. Department of Disease Control and Homeostasis, Kanazawa University Hospital, Kanazawa, Japan. 5. Department of Laboratory Sciences, School of Health Sciences, Kanazawa University, Kanazawa, Japan.
Abstract
BACKGROUND: Various sleep abnormalities, such as delayed sleep onset, frequent awakening and daytime sleepiness, deteriorate the quality of life in patients with chronic kidney disease (CKD), including those on haemodialysis (HD). Although there are some candidate causative molecules in the central nervous system, the contribution of peripheral blood cells (PBCs) remains unclear. In this study, we performed polysomnographic analysis in CKD patients and used PBCs to examine the expression of genes related to sleep and wakefulness states. METHODS: Polysomnographic analysis was performed in 9 CKD patients and 6 controls. Genes related to sleep and wakefulness were evaluated by RNA microarray in 19 subjects, including CKD patients and control subjects. RESULTS: Polysomnographic analysis revealed that the duration of the rapid eye movement (REM)/non-REM phases during total sleep time was different between CKD patients and healthy controls. In mRNA microarray evaluation, hierarchical clustering analysis showed different patterns of sleep-related gene expression in HD patients. mRNA expression levels of GABA receptor (GABBR2), noradrenaline receptor (ADRA1A), dopamine receptor (DRD1) and histamine receptor (HRH1) showed an inverse correlation with renal function. Moreover, the mRNA expression of orexin and its receptor (HCRTR1 and HCRTR2) was also inversely correlated with renal function. CONCLUSION: These data indicate that the expression of sleep-related genes in PBCs of CKD patients may be associated with sleep abnormalities.
BACKGROUND: Various sleep abnormalities, such as delayed sleep onset, frequent awakening and daytime sleepiness, deteriorate the quality of life in patients with chronic kidney disease (CKD), including those on haemodialysis (HD). Although there are some candidate causative molecules in the central nervous system, the contribution of peripheral blood cells (PBCs) remains unclear. In this study, we performed polysomnographic analysis in CKDpatients and used PBCs to examine the expression of genes related to sleep and wakefulness states. METHODS: Polysomnographic analysis was performed in 9 CKDpatients and 6 controls. Genes related to sleep and wakefulness were evaluated by RNA microarray in 19 subjects, including CKDpatients and control subjects. RESULTS: Polysomnographic analysis revealed that the duration of the rapid eye movement (REM)/non-REM phases during total sleep time was different between CKDpatients and healthy controls. In mRNA microarray evaluation, hierarchical clustering analysis showed different patterns of sleep-related gene expression in HDpatients. mRNA expression levels of GABA receptor (GABBR2), noradrenaline receptor (ADRA1A), dopamine receptor (DRD1) and histamine receptor (HRH1) showed an inverse correlation with renal function. Moreover, the mRNA expression of orexin and its receptor (HCRTR1 and HCRTR2) was also inversely correlated with renal function. CONCLUSION: These data indicate that the expression of sleep-related genes in PBCs of CKDpatients may be associated with sleep abnormalities.
Authors: Sharon Wallace Williams; Grethe S Tell; Beiyao Zheng; Sally Shumaker; Michael V Rocco; Mary Ann Sevick Journal: Am J Nephrol Date: 2002 Jan-Feb Impact factor: 3.754
Authors: T Sakurai; A Amemiya; M Ishii; I Matsuzaki; R M Chemelli; H Tanaka; S C Williams; J A Richardson; G P Kozlowski; S Wilson; J R Arch; R E Buckingham; A C Haynes; S A Carr; R S Annan; D E McNulty; W S Liu; J A Terrett; N A Elshourbagy; D J Bergsma; M Yanagisawa Journal: Cell Date: 1998-02-20 Impact factor: 41.582