Literature DB >> 26387819

Identification of a Novel Small Molecule Inhibitor Against SARS Coronavirus Helicase.

Jin-Beom Cho1, Jin-Moo Lee1, Hee-Chul Ahn2, Yong-Joo Jeong1.   

Abstract

A new chemical inhibitor against severe acute respiratory syndrome (SARS) coronavirus helicase, 7-ethyl-8-mercapto-3-methyl-3,7-dihydro-1H-purine-2,6-dione, was identified. We investigated the inhibitory effect of the compound by conducting colorimetry-based ATP hydrolysis assay and fluorescence resonance energy transfer-based double-stranded DNA unwinding assay. The compound suppressed both ATP hydrolysis and double-stranded DNA unwinding activities of helicase with IC50 values of 8.66 ± 0.26 μM and 41.6 ± 2.3 μM, respectively. Moreover, we observed that the compound did not show cytotoxicity up to 80 µMconcentration. Our results suggest that the compound might serve as a SARS coronavirus inhibitor.

Entities:  

Keywords:  ATP hydrolysis; SARS; dsDNA unwinding; helicase

Mesh：

Substances：

Year:  2015        PMID： 26387819     DOI： 10.4014/jmb.1507.07078

Source DB:  PubMed          Journal:  J Microbiol Biotechnol        ISSN： 1017-7825            Impact factor:   2.351


  9 in total

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Authors:  Ruixuan Wang; Preyesh Stephen; Yi Tao; Wenfa Zhang; Sheng-Xiang Lin
Journal:  Biomed Pharmacother       Date:  2021-01-13       Impact factor: 7.419

Review 5.  New insights into application of nanoparticles in the diagnosis and screening of novel coronavirus (SARS-CoV-2).

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6.  Coronavirus helicases: attractive and unique targets of antiviral drug-development and therapeutic patents.

Authors:  Austin N Spratt; Fabio Gallazzi; Thomas P Quinn; Christian L Lorson; Anders Sönnerborg; Kamal Singh
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Authors:  Jinglei Xu; Haiyan Huang; Xiang Zhou
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Authors:  Namrta Choudhry; Xin Zhao; Dan Xu; Mark Zanin; Weisan Chen; Zifeng Yang; Jianxin Chen
Journal:  J Med Chem       Date:  2020-09-11       Impact factor: 7.446

9.  Structure-Based Virtual Screening Identifies Multiple Stable Binding Sites at the RecA Domains of SARS-CoV-2 Helicase Enzyme.

Authors:  Sajjad Ahmad; Yasir Waheed; Saba Ismail; Saadia Bhatti; Sumra Wajid Abbasi; Khalid Muhammad
Journal:  Molecules       Date:  2021-03-07       Impact factor: 4.411
  9 in total

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