| Literature DB >> 26385361 |
Li Wei1, Hanmiao Sheng2, Long Chen1, Bin Hao3, Xiaohong Shi4, Yinghui Chen1.
Abstract
We investigated the effect of pannexin-1 (PANX-1) on the release of glutamate and cytokines in U87 human malignant glioma (U87-MG) cells using small interfering RNA (siRNA) transfection and adenosine triphosphate/lipopolysaccharide treatment. PANX-1 is a new family member of the gap junction proteins, and is permeable to ions, metabolic molecules, second messengers, and neurotransmitters, among other factors. PANX-1 plays an important role in the regulation of cell inflammation. However, the relationship between PANX-1 and the secretion of glutamate, interleukin (IL)-6 and IL-8 in astrocytes is still poorly understood. We found that the levels of IL-6, IL-8 and glutamate were lower in PANX-1 siRNA transfected cells, while the levels of IL-6, IL-8 and glutamate were unaltered in cells without changes in the expression of PANX-1 protein. This provides further support for the hypothesis that PANX-1 plays an important role in the release of IL-6, IL-8 and glutamate in U87-MG cells.Entities:
Keywords: Astrocytes; Cytokine; Gap junction; Glutamate; Pannexin-1
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Year: 2015 PMID： 26385361 DOI： 10.1016/j.jocn.2015.05.043
Source DB: PubMed Journal: J Clin Neurosci ISSN： 0967-5868 Impact factor: 1.961