Yang Yu1,2,3, Qian Gao1, Hong-Cui Zhao1, Rong Li1,2,3, Jiang-Man Gao1, Ting Ding1,3, Si-Yu Bao1,2, Yue Zhao1,3, Xiao-Fang Sun4, Yong Fan5,6, Jie Qiao7,8,9. 1. Department of Obstetrics and Gynecology, Center of Reproductive Medicine, Peking University Third Hospital, No. 49 HuaYuan North Road, HaiDian District, Beijing, 100191, People's Republic of China. 2. Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, 100191, China. 3. Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, 100191, China. 4. Key Laboratory for Major Obstetric Diseases of Guangdong Province, the Third Affiliated Hospital of Guangzhou Medical University, No. 63, Liwan District, Guangzhou City, 510150, Guangdong Province, People's Republic of China. 5. Department of Obstetrics and Gynecology, Center of Reproductive Medicine, Peking University Third Hospital, No. 49 HuaYuan North Road, HaiDian District, Beijing, 100191, People's Republic of China. fanyong0413@sina.com. 6. Key Laboratory for Major Obstetric Diseases of Guangdong Province, the Third Affiliated Hospital of Guangzhou Medical University, No. 63, Liwan District, Guangzhou City, 510150, Guangdong Province, People's Republic of China. fanyong0413@sina.com. 7. Department of Obstetrics and Gynecology, Center of Reproductive Medicine, Peking University Third Hospital, No. 49 HuaYuan North Road, HaiDian District, Beijing, 100191, People's Republic of China. jie.qiao@263.net. 8. Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, 100191, China. jie.qiao@263.net. 9. Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, 100191, China. jie.qiao@263.net.
Following the publication of our article [1], we noticed that some incorrect images had been incorporated into figure twoB (included here as Fig. 1b) and threeF-H (included here as Fig. 2f-h) in error. The corrected figures are given below. This correction does not change the results or conclusion of the original study.
Fig. 1
Identification of human parthenogenetic embryonic stem cells. a Colony of human parthenogenetic embryonic stem cells; (b) positive staining for alkaline phosphatase; (c) normal 46, XX karyotype at passage 20; (d) positive staining for OCT4; (e) positive staining for NANOG; (f) positive staining for TRA-1-60; (D1-F1) nuclear staining with Hoechst 33342; (D2-F2) merged images for OCT4, NANOG and TRA-1-60. Bar is 100 μm
Fig. 2
Differentiation abilities of human parthenogenetic embryonic stem cells. In vitro differentiated EBs displayed (a) positive AFP staining (endoderm), (b) positive SMA staining (mesoderm), (c) positive TUBULIN staining (ectoderm), and (d) expression of genes from endoderm (NF68KD), mesoderm (HBZ) and ectoderm (Albumin). Bar is 50 μm. (e) Efficiency of teratoma formation upon injection of human parthenogenetic embryonic stem cells into SCID mice; (f) neuro-ectoderm from ectoderm in teratoma; (g) cartilage from mesoderm in teratoma; (h) glandular tissue from endoderm in teratoma. Bar is 100 μm. EB, embryoid bodies; SCID, severe combined immunodeficiency
Identification of human parthenogenetic embryonic stem cells. a Colony of human parthenogenetic embryonic stem cells; (b) positive staining for alkaline phosphatase; (c) normal 46, XX karyotype at passage 20; (d) positive staining for OCT4; (e) positive staining for NANOG; (f) positive staining for TRA-1-60; (D1-F1) nuclear staining with Hoechst 33342; (D2-F2) merged images for OCT4, NANOG and TRA-1-60. Bar is 100 μmDifferentiation abilities of human parthenogenetic embryonic stem cells. In vitro differentiated EBs displayed (a) positive AFP staining (endoderm), (b) positive SMA staining (mesoderm), (c) positive TUBULIN staining (ectoderm), and (d) expression of genes from endoderm (NF68KD), mesoderm (HBZ) and ectoderm (Albumin). Bar is 50 μm. (e) Efficiency of teratoma formation upon injection of human parthenogenetic embryonic stem cells into SCIDmice; (f) neuro-ectoderm from ectoderm in teratoma; (g) cartilage from mesoderm in teratoma; (h) glandular tissue from endoderm in teratoma. Bar is 100 μm. EB, embryoid bodies; SCID, severe combined immunodeficiency