Lee R Katzman1, Caroline K Hoover, Yousuf M Khalifa, Bennie H Jeng. 1. *Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD; †SightLife, Seattle, WA; ‡Department of Ophthalmology, Grady Memorial Hospital, Atlanta, GA; and §Department of Ophthalmology, Emory University, Atlanta, GA.
Abstract
PURPOSE: To evaluate the accuracy of eye bank-prepared precut donor corneas over time by comparing cut-failure rates and corneal thickness measurements in 2010 and 2013. METHODS: A total of 2511 human corneas cut by a technician-operated mechanical microkeratome intended for endothelial keratoplasty were evaluated prospectively at one large eye bank facility in 2010 and in 2013. The endothelium was evaluated by slit lamp, and specular microscopy both before and after cutting was performed. Graft thickness as measured by pachymetry and/or optical coherence tomography was collected to assess the accuracy of the cut tissue. Cut-failure rates were compared between normal donor tissue and tissue with significant preexisting scarring. RESULTS: The combined cut-failure rate in 2010 and 2013 was 2.3% (23/1000) and 1.6% (24/1511), respectively (P = 0.23). The cut-failure rate among normal tissue in 2010 and 2013 was 2.0% (19/927) and 1.4% (19/1400), respectively (P = 0.24). The cut-failure rate among previously scarred tissue in 2010 and 2013 was 5.5% (4/73) and 4.5% (5/111), respectively (P = 0.74). The mean surgeon-requested graft thickness was 144.7 μm (range 100-150, SD 13.6) and 127.2 μm (range 75-150, SD 25.2) in 2010 and 2013, respectively (P < 0.0001). The mean deviation from target graft thickness was 21.3 μm (SD 16.3) and 13.6 μm (SD 12.5) in 2010 and 2013, respectively (P < 0.0001). CONCLUSIONS: From 2010 to 2013, the combined cut-failure rates trended toward improvement, while the accuracy of graft thickness improved. This study suggests that the accuracy and success rates of tissue preparation for endothelial keratoplasty improve with experience and volume.
PURPOSE: To evaluate the accuracy of eye bank-prepared precut donor corneas over time by comparing cut-failure rates and corneal thickness measurements in 2010 and 2013. METHODS: A total of 2511 human corneas cut by a technician-operated mechanical microkeratome intended for endothelial keratoplasty were evaluated prospectively at one large eye bank facility in 2010 and in 2013. The endothelium was evaluated by slit lamp, and specular microscopy both before and after cutting was performed. Graft thickness as measured by pachymetry and/or optical coherence tomography was collected to assess the accuracy of the cut tissue. Cut-failure rates were compared between normal donor tissue and tissue with significant preexisting scarring. RESULTS: The combined cut-failure rate in 2010 and 2013 was 2.3% (23/1000) and 1.6% (24/1511), respectively (P = 0.23). The cut-failure rate among normal tissue in 2010 and 2013 was 2.0% (19/927) and 1.4% (19/1400), respectively (P = 0.24). The cut-failure rate among previously scarred tissue in 2010 and 2013 was 5.5% (4/73) and 4.5% (5/111), respectively (P = 0.74). The mean surgeon-requested graft thickness was 144.7 μm (range 100-150, SD 13.6) and 127.2 μm (range 75-150, SD 25.2) in 2010 and 2013, respectively (P < 0.0001). The mean deviation from target graft thickness was 21.3 μm (SD 16.3) and 13.6 μm (SD 12.5) in 2010 and 2013, respectively (P < 0.0001). CONCLUSIONS: From 2010 to 2013, the combined cut-failure rates trended toward improvement, while the accuracy of graft thickness improved. This study suggests that the accuracy and success rates of tissue preparation for endothelial keratoplasty improve with experience and volume.
Authors: Kevin W Ross; Christopher G Stoeger; George O D Rosenwasser; Robert C OʼBrien; Loretta B Szczotka-Flynn; Allison R Ayala; Maureen G Maguire; Beth Ann Benetz; Patricia Dahl; Donna C Drury; Steven P Dunn; Sameera M Farazdaghi; Caroline K Hoover; Marian S Macsai; Shahzad I Mian; Michael L Nordlund; Jeffrey G Penta; Mark C Soper; Mark A Terry; David D Verdier; Doyce V Williams; Jonathan H Lass Journal: Cornea Date: 2019-09 Impact factor: 3.152