Bo Liu1, Zhicheng Yao1, Kunpeng Hu1, He Huang1, Shilei Xu1, Qinliang Wang1, Yang Yang2, Jie Ren3. 1. Department of general surgery, the third affiliated hospital, Sun Yat-Sen University, Guangzhou 510530, China. 2. Department of liver transplantation, the third affiliated hospital, Sun Yat-Sen University, Guangzhou 510530, China. 3. Department of ultrasound, the third affiliated hospital, Sun Yat-Sen University, Guangzhou 510530, China. Electronic address: renjie150421@163.com.
Abstract
BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common and lethal malignancies in the world, and hepatitis B virus (HBV) has been well established to cause HCC. Ndc80 complex is a conserved mitotic regulator dedicated to ensuring faithful chromosome segregation and plays an important role in inducing tumor formation. However, its role in HCC caused by HBV infection remains unclear. METHODS: Immunohistochemistry (IHC), Western blot (WB), and real-time qRT-PCR were used to measure the expression of Ndc80 in HBV-related HCC tissues. Ndc80-specific short hairpin RNA (shRNA) was used to knock-down Ndc80 expression in the hepatoma cell line HeG2 and HepG2.2.15, which is stable transcribed with HBV genome. Furthermore, the effect of Ndc80 on cellular proliferation and growth were examined, respectively. RESULTS: The expression level of Ndc80 was remarkably up-regulated in HBV-related HCC tissues. Down-regulation of Ndc80 expression suppressed HBV replication. With cell counting and the MTS assay, cellular proliferation and growth of Ndc80 knocking-down cell line was shown to be effectively restrained. CONCLUSION: This study suggests that Ndc80 may play an important role in the process of HBV-related HCC, and that it may be a potential biological treatment target in the future.
BACKGROUND:Hepatocellular carcinoma (HCC) is one of the most common and lethal malignancies in the world, and hepatitis B virus (HBV) has been well established to cause HCC. Ndc80 complex is a conserved mitotic regulator dedicated to ensuring faithful chromosome segregation and plays an important role in inducing tumor formation. However, its role in HCC caused by HBV infection remains unclear. METHODS: Immunohistochemistry (IHC), Western blot (WB), and real-time qRT-PCR were used to measure the expression of Ndc80 in HBV-related HCC tissues. Ndc80-specific short hairpin RNA (shRNA) was used to knock-down Ndc80 expression in the hepatoma cell line HeG2 and HepG2.2.15, which is stable transcribed with HBV genome. Furthermore, the effect of Ndc80 on cellular proliferation and growth were examined, respectively. RESULTS: The expression level of Ndc80 was remarkably up-regulated in HBV-related HCC tissues. Down-regulation of Ndc80 expression suppressed HBV replication. With cell counting and the MTS assay, cellular proliferation and growth of Ndc80 knocking-down cell line was shown to be effectively restrained. CONCLUSION: This study suggests that Ndc80 may play an important role in the process of HBV-related HCC, and that it may be a potential biological treatment target in the future.
Authors: Nahid H Hajrah; Waseem Mohammed Abdul; Zainab H Abdul-Hameed; Walied M Alarif; Nouf Saeed A Al-Abbas; Seif-Eldin N Ayyad; Abdulkader M Shaikh Omer; Mohammed Zainy Mutawakil; Neil Hall; Abdullah Y Obaid; Roop Singh Bora; Jamal S M Sabir; Kulvinder Singh Saini Journal: Integr Cancer Ther Date: 2020 Jan-Dec Impact factor: 3.279