Gang Xu1,2, Yu Jiang3,4, Rongshen Tao5, Shengfeng Wang2, Hongyu Zeng2, Sheng Yang6,7. 1. College of Life Science and Technology, Central South University of Forestry and Technology, Changsha, 410004, China. 2. Hunan Flag Bio-tech Co. Ltd, Changsha, 410100, China. 3. Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China. 4. Shanghai Collaborative Innovation Center for Biomanufacturing Technology, Shanghai, 200237, China. 5. Huzhou Research Center of Industrial Biotechnology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Huzhou, 313000, China. 6. Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China. syang@sibs.ac.cn. 7. Shanghai Collaborative Innovation Center for Biomanufacturing Technology, Shanghai, 200237, China. syang@sibs.ac.cn.
Abstract
OBJECTIVE: To make the previously developed biosynthesis of L-2-aminobutyric acid (L-ABA) more suitable for the industrial-scale production. RESULTS: A recyclable biotransformation system was developed based on immobilized enzyme technology. The conversion yield of L-threonine (at 90 g l(-1)) reached 99.9 % and the theoretical yield of L-ABA reached more than 90 % using the optimized biotransformation system by the individual immobilization of threonine deaminase and the co-immobilization of L leucine dehydrogenase and formate dehydrogenase. 90 g L-threonine l(-1) was converted to 73.9 g L-ABA l(-1) >95 % theoretical yield, within 120-145 min in 30 batch transformation experiments. CONCLUSION: The recyclable biotransformation system is promising to fulfill industrial requirements for L-ABA production.
OBJECTIVE: To make the previously developed biosynthesis of L-2-aminobutyric acid (L-ABA) more suitable for the industrial-scale production. RESULTS: A recyclable biotransformation system was developed based on immobilized enzyme technology. The conversion yield of L-threonine (at 90 g l(-1)) reached 99.9 % and the theoretical yield of L-ABA reached more than 90 % using the optimized biotransformation system by the individual immobilization of threonine deaminase and the co-immobilization of L leucine dehydrogenase and formate dehydrogenase. 90 g L-threonine l(-1) was converted to 73.9 g L-ABA l(-1) >95 % theoretical yield, within 120-145 min in 30 batch transformation experiments. CONCLUSION: The recyclable biotransformation system is promising to fulfill industrial requirements for L-ABA production.