Literature DB >> 263737

A bioassay capable of measuring 1 picogram of 1,25-dihydroxyvitamin D3.

P H Stern, A J Hamstra, H F DeLuca, N H Bell.   

Abstract

Fetal rat bones in organ culture constitute a sensitive system for assay of the vitamin D metabolite, 1 alpha,25-dihydroxyvitamin D3. Significant bone resorption is obtained with as little as 2 pg 1,25-dihydroxyvitamin D3 after 48 h of culture and with 1 pg after 64 h of culture. In the current study, organ cultures of fetal rat bone are used as a bioassay for 1,25-dihydroxyvitamin D i normal human plasma, which was prepared for assay by extraction with dichloromethane, chromatography on Sephadex LH-20, and purification on silicic acid by high pressure liquid chromatography. The concentration of 1 alpha,25-dihydroxyvitamin D3 in normal adult human plasma was 24.8 +/- 2.0 pg/ml (n = 19) by this assay.

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Year:  1978        PMID： 263737     DOI： 10.1210/jcem-46-6-891

Source DB:  PubMed          Journal:  J Clin Endocrinol Metab        ISSN： 0021-972X            Impact factor:   5.958


  7 in total

1.  Transport of vitamin D: significance of free and total concentrations of the vitamin D metabolites.

Authors:  R Bouillon; H Van Baelen
Journal:  Calcif Tissue Int       Date:  1981       Impact factor: 4.333

2.  A competitive protein binding assay for 1, 25-dihydroxy vitamin D in blood.

Authors:  M W France; B Lalor
Journal:  Ir J Med Sci       Date:  1981-10       Impact factor: 1.568

3.  Evidence that increased circulating 1 alpha, 25-dihydroxyvitamin D is the probable cause for abnormal calcium metabolism in sarcoidosis.

Authors:  N H Bell; P H Stern; E Pantzer; T K Sinha; H F DeLuca
Journal:  J Clin Invest       Date:  1979-07       Impact factor: 14.808

4.  Do tissues other than the kidney produce 1,25-dihydroxyvitamin D3 in vivo? A reexamination.

Authors:  T D Shultz; J Fox; H Heath; R Kumar
Journal:  Proc Natl Acad Sci U S A       Date:  1983-03       Impact factor: 11.205

5.  Comparison of fetal rat limb bones and neonatal mouse calvaria: effects of parathyroid hormone and 1,25-dihydroxyvitamin D3.

Authors:  P H Stern; N S Krieger
Journal:  Calcif Tissue Int       Date:  1983       Impact factor: 4.333

6.  Isolation of osteoclasts from fetal rat long bones.

Authors:  T J Hefley; P H Stern
Journal:  Calcif Tissue Int       Date:  1982-09       Impact factor: 4.333

7.  Evidence for abnormal regulation of circulating 1 alpha,25-dihydroxyvitamin D in patients with sarcoidosis and normal calcium metabolism.

Authors:  P H Stern; J De Olazabal; N H Bell
Journal:  J Clin Invest       Date:  1980-10       Impact factor: 14.808
  7 in total

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