Robert De Matteo1, Noreen Ishak1, Takushi Hanita1,2, Richard Harding1, Foula Sozo1. 1. Development and Stem Cells Program, Monash Biomedicine Discovery Institute and Department of Anatomy and Developmental Biology, Monash University, Melbourne, Australia. 2. Department of Pediatrics, Tohoku University School of Medicine, Aobaku, Sendai, Japan.
Abstract
BACKGROUND: Male preterm infants are more likely to experience respiratory distress syndrome than females. Our objectives were to determine if sex-related differences in physiological adaptation after preterm birth increase with time after birth and if the use of continuous positive airway pressure (CPAP) reduces these differences. METHODS: Unanesthetized lambs (9F, 8M) were delivered at 0.90 of term. Blood gases, metabolites, and cardiovascular and respiratory parameters were monitored in spontaneously breathing lambs for 8 h. Supplemental oxygen was administered via a face mask at 4 cmH2O CPAP. At 8 h, lung compliance was determined, and bronchoalveolar lavage fluid (BALF) was analyzed for total protein and surfactant phospholipids. Surfactant protein (SP) gene expression and protein expression of SP-A and pro-SP-C were determined in lung tissue. RESULTS: For 8 h after delivery, males had significantly lower arterial pH and higher Paco2, and a greater percentage of males were dependent on supplemental oxygen than females. Inspiratory effort was greater and lung compliance was lower in male lambs. Total protein concentration in BALF, SP gene expression, and SP-A protein levels were not different between sexes; pro-SP-C was 24% lower in males. CONCLUSION: The use of CPAP did not eliminate the male disadvantage, which continues for up to 8 h after preterm birth.
BACKGROUND: Male preterm infants are more likely to experience respiratory distress syndrome than females. Our objectives were to determine if sex-related differences in physiological adaptation after preterm birth increase with time after birth and if the use of continuous positive airway pressure (CPAP) reduces these differences. METHODS: Unanesthetized lambs (9F, 8M) were delivered at 0.90 of term. Blood gases, metabolites, and cardiovascular and respiratory parameters were monitored in spontaneously breathing lambs for 8 h. Supplemental oxygen was administered via a face mask at 4 cmH2O CPAP. At 8 h, lung compliance was determined, and bronchoalveolar lavage fluid (BALF) was analyzed for total protein and surfactant phospholipids. Surfactant protein (SP) gene expression and protein expression of SP-A and pro-SP-C were determined in lung tissue. RESULTS: For 8 h after delivery, males had significantly lower arterial pH and higher Paco2, and a greater percentage of males were dependent on supplemental oxygen than females. Inspiratory effort was greater and lung compliance was lower in male lambs. Total protein concentration in BALF, SP gene expression, and SP-A protein levels were not different between sexes; pro-SP-C was 24% lower in males. CONCLUSION: The use of CPAP did not eliminate the male disadvantage, which continues for up to 8 h after preterm birth.
Authors: Foula Sozo; Melissa Vela; Victoria Stokes; Kelly Kenna; Peter J Meikle; Robert De Matteo; David Walker; James Brien; Alan Bocking; Richard Harding Journal: Am J Physiol Lung Cell Mol Physiol Date: 2010-10-29 Impact factor: 5.464
Authors: Jeroen J van Vonderen; Stuart B Hooper; Helmut D Hummler; Enrico Lopriore; Arjan B te Pas Journal: J Pediatr Date: 2014-07-16 Impact factor: 4.406
Authors: Marshall M Mrocki; Vivian B Nguyen; Paul Lombardo; Megan R Sutherland; Jonathan G Bensley; Ilias Nitsos; Beth J Allison; Richard Harding; Robert De Matteo; Michal Schneider; Graeme R Polglase; M Jane Black Journal: J Physiol Date: 2018-04-06 Impact factor: 5.182