OBJECTIVE: To examine interleukin-12 (IL-12), IL-18, IFN-γ, intracellular adhesion molecule-1 (ICAM-1), leukemia inhibitory factor (LIF), and migration inhibitory factor (MIF) levels in precisely-timed blood and endometrial tissue samples from women with idiopathic recurrent pregnancy loss (RPL). DESIGN: Case-control study. SETTING: University hospital. PATIENT(S): Twenty-one women with RPL and 20 women with proven fertility (controls). INTERVENTION(S): Primary endometrial cells and blood samples during the midsecretory phase (days 19-23). MAIN OUTCOME MEASURE(S): Detection of IL-12, IL-18, IFN-γ, ICAM-1, LIF, and MIF via enzyme-linked immunosorbent assay in both blood and endometrial tissue samples. RESULT(S): The blood and tissue levels of IL-12, IL-18, and IFN-γ were statistically significantly higher, and the blood and tissue levels of LIF and MIF were statistically significantly lower in patients with RPL. Only the level of tissue ICAM-1 was higher in patients with RPL. There was a strong correlation between blood and tissue level measurements of IL-12, IL-18, LIF, and MIF. CONCLUSION(S): Our findings support the hypothesis that inflammatory processes may contribute to pregnancy loss, possibly through their role in implantation. We found that blood and tissue levels of IL-18, LIF, and MIF, and tissue levels of IL-12, IFN-γ, and ICAM-1 have statistically significant prognostic relevance.
OBJECTIVE: To examine interleukin-12 (IL-12), IL-18, IFN-γ, intracellular adhesion molecule-1 (ICAM-1), leukemia inhibitory factor (LIF), and migration inhibitory factor (MIF) levels in precisely-timed blood and endometrial tissue samples from women with idiopathic recurrent pregnancy loss (RPL). DESIGN: Case-control study. SETTING: University hospital. PATIENT(S): Twenty-one women with RPL and 20 women with proven fertility (controls). INTERVENTION(S): Primary endometrial cells and blood samples during the midsecretory phase (days 19-23). MAIN OUTCOME MEASURE(S): Detection of IL-12, IL-18, IFN-γ, ICAM-1, LIF, and MIF via enzyme-linked immunosorbent assay in both blood and endometrial tissue samples. RESULT(S): The blood and tissue levels of IL-12, IL-18, and IFN-γ were statistically significantly higher, and the blood and tissue levels of LIF and MIF were statistically significantly lower in patients with RPL. Only the level of tissue ICAM-1 was higher in patients with RPL. There was a strong correlation between blood and tissue level measurements of IL-12, IL-18, LIF, and MIF. CONCLUSION(S): Our findings support the hypothesis that inflammatory processes may contribute to pregnancy loss, possibly through their role in implantation. We found that blood and tissue levels of IL-18, LIF, and MIF, and tissue levels of IL-12, IFN-γ, and ICAM-1 have statistically significant prognostic relevance.
Authors: Michael R Strug; Ren-Wei Su; Tae Hoon Kim; Alessandro Mauriello; Carlo Ticconi; Bruce A Lessey; Steven L Young; Jeong Mook Lim; Jae-Wook Jeong; Asgerally T Fazleabas Journal: FASEB J Date: 2018-01-08 Impact factor: 5.191
Authors: R G Radin; S L Mumford; L A Sjaarda; R M Silver; J Wactawski-Wende; A M Lynch; N J Perkins; L L Lesher; B D Wilcox; S N Hinkle; T C Plowden; K Kim; E F Schisterman Journal: BJOG Date: 2017-10-25 Impact factor: 6.531
Authors: Kasra Khalaj; Rayana Leal Luna; Maria Eduarda Rocha de França; Wilma Helena de Oliveira; Christina Alves Peixoto; Chandrakant Tayade Journal: Oncotarget Date: 2016-11-08