Tekin Guney1, Murat Alisik2, Afra Alkan3, Abdulkadir Basturk4, Sema Akinci5, Tuba Hacibekiroglu6, Imdat Dilek7, Ozcan Erel8. 1. a Department of Hematology , Turkiye Yuksek Ihtisas Training and Research Hospital , Ankara , Turkey. 2. b Department of Medical Biochemistry , Ataturk Training and Research Hospital , Ankara , Turkey. 3. c Department of Biostatistics and Medical Informatics , Yildirim Beyazit University Faculty of Medicine , Ankara , Turkey. 4. d Department of Hematology , Konya Training and Research Hospital , Konya , Turkey. 5. e Department of Hematology , Ataturk Training and Research Hospital , Ankara , Turkey. 6. f Department of Hematology , Sakarya Training and Research Hospital , Sakarya , Turkey. 7. g Department of Hematology , Yildirim Beyazit University Faculty of Medicine , Ankara , Turkey. 8. h Department of Medical Biochemistry , Yildirim Beyazit University Faculty of Medicine , Ankara , Turkey.
Abstract
OBJECTIVE: The purpose of this study was to investigate paraoxonase (PON) and arylesterase (ARES) enzyme activity in adults with vitamin B12 deficiency, and specific changes in the activities of these enzymes following vitamin B12 treatment. METHODS: A total of 46 patients with vitamin B12 deficiency (aged 18-82 years) and 45 healthy volunteer controls (aged 19-64 years) participated in this study. Venous blood samples were collected, and serum vitamin B12, homocysteine (HCY), methylmalonic acid, PON1, and ARES levels were measured. RESULTS: Paired comparison showed that pre- and post-treatment values for PON and ARES were similar between patients and controls (both P > 0.05). There was no statistically significant relationship between patients' pre-/post-treatment PON or HCY levels and serum vitamin B12 levels, compared with those of the control group (P > 0.05). DISCUSSION: The results of the present study do not support the hypothesis that the antioxidant enzymes PON and ARES have an underlying role in vitamin B12 deficiency and related hyperhomocysteinemia. Our findings suggest that PON and ARES do not play a role in the systemic effects of vitamin B12 deficiency.
OBJECTIVE: The purpose of this study was to investigate paraoxonase (PON) and arylesterase (ARES) enzyme activity in adults with vitamin B12 deficiency, and specific changes in the activities of these enzymes following vitamin B12 treatment. METHODS: A total of 46 patients with vitamin B12 deficiency (aged 18-82 years) and 45 healthy volunteer controls (aged 19-64 years) participated in this study. Venous blood samples were collected, and serum vitamin B12, homocysteine (HCY), methylmalonic acid, PON1, and ARES levels were measured. RESULTS: Paired comparison showed that pre- and post-treatment values for PON and ARES were similar between patients and controls (both P > 0.05). There was no statistically significant relationship between patients' pre-/post-treatment PON or HCY levels and serum vitamin B12 levels, compared with those of the control group (P > 0.05). DISCUSSION: The results of the present study do not support the hypothesis that the antioxidant enzymes PON and ARES have an underlying role in vitamin B12 deficiency and related hyperhomocysteinemia. Our findings suggest that PON and ARES do not play a role in the systemic effects of vitamin B12 deficiency.