Tianxiao Li1, Zhimin Ren2, Ying Deng3, Yi Wang4, Hongbo Zhou1. 1. a Department of Biochemistry and Molecular Biology , Harbin Medical University , Harbin , China . 2. b Department of Pediatrics and. 3. c Department of Emergency , The Second Affiliated Hospital, Harbin Medical University , Harbin , China , and. 4. d Biotechnology Experimental Teaching Center, Harbin Medical University , Harbin , China.
Abstract
OBJECTIVE: RAD50-IL13 region has been recently identified as one of critical asthma susceptibility loci in genome-wide association studies, yet the role of these genetic variants or single-nucleotide polymorphisms (SNPs) in the risk of developing asthma in Northeastern Han Chinese remains largely unknown. METHODS: We conducted an association study by genotyping four SNPs (rs2244012 and rs6871536 in RAD50 as well as rs1295686 and rs1800925 in IL13) in 652 asthmatic children and age-matched 752 healthy controls from Northeastern Han Chinese to evaluate the asthma susceptibility with each individual SNP using SNaPshot genotyping method. RESULTS: We did not find the allele or genotype frequency distribution of four SNPs in RAD50-IL13 region which was significantly different between asthmatic children and controls (p > 0.05). CONCLUSIONS: Our findings first suggested that the variants in RAD50-IL13 region were not associated with asthma risk in Northeastern Han Chinese children.
OBJECTIVE:RAD50-IL13 region has been recently identified as one of critical asthma susceptibility loci in genome-wide association studies, yet the role of these genetic variants or single-nucleotide polymorphisms (SNPs) in the risk of developing asthma in Northeastern Han Chinese remains largely unknown. METHODS: We conducted an association study by genotyping four SNPs (rs2244012 and rs6871536 in RAD50 as well as rs1295686 and rs1800925 in IL13) in 652 asthmatic children and age-matched 752 healthy controls from Northeastern Han Chinese to evaluate the asthma susceptibility with each individual SNP using SNaPshot genotyping method. RESULTS: We did not find the allele or genotype frequency distribution of four SNPs in RAD50-IL13 region which was significantly different between asthmatic children and controls (p > 0.05). CONCLUSIONS: Our findings first suggested that the variants in RAD50-IL13 region were not associated with asthma risk in Northeastern Han Chinese children.
Entities:
Keywords:
Asthma; Han Chinese children; IL13; RAD50; single-nucleotide polymorphism
Authors: Enrique Ambrocio-Ortiz; Gustavo Galicia-Negrete; Gloria Pérez-Rubio; Areli J Escobar-Morales; Edgar Abarca-Rojano; Alma D Del Angel-Pablo; Manuel D J Castillejos-López; Ramcés Falfán-Valencia Journal: Diagnostics (Basel) Date: 2020-04-30
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