Literature DB >> 26365227

Practical and low cost whole-organism motility assay: A step-by-step protocol.

Sarah Preston1, Abdul Jabbar1, Cameron Nowell2, Anja Joachim3, Baerbel Ruttkowski3, Tony Cardno2, Andreas Hofmann4, Robin B Gasser5.   

Abstract

Here, we provide a step-by-step protocol for a practical and low cost whole-organism assay for the screening of chemical compounds for activity against parasitic worms. This assay has considerable advantages over conventional methods, mainly in relation to ease of use, throughput, time and cost. It is readily suited to the screening of hundreds to thousands of compounds for subsequent hit-to-lead optimisation, and should be applicable to many different parasites and other organisms commensurate with the size of wells in the microtiter plates used for phenotypic screening.
Copyright © 2015 Elsevier Ltd. All rights reserved.

Keywords:  Drug screening; Motility assay; Nematode; Parasite; Step-by-step protocol

Mesh：

Substances：

Year:  2015        PMID： 26365227     DOI： 10.1016/j.mcp.2015.08.005

Source DB:  PubMed          Journal:  Mol Cell Probes        ISSN： 0890-8508            Impact factor:   2.365


  11 in total

1.  Image recognition based on deep learning in Haemonchus contortus motility assays.

Authors:  Martin Žofka; Linh Thuy Nguyen; Eva Mašátová; Petra Matoušková
Journal:  Comput Struct Biotechnol J       Date:  2022-05-13       Impact factor: 6.155

2.  Selenophene and thiophene-core estrogen receptor ligands that inhibit motility and development of parasitic stages of Haemonchus contortus.

Authors:  Sarah Preston; Junjie Luo; Yuezhou Zhang; Abdul Jabbar; Simon Crawford; Jonathan Baell; Andreas Hofmann; Min Hu; Hai-Bing Zhou; Robin B Gasser
Journal:  Parasit Vectors       Date:  2016-06-16       Impact factor: 3.876

3.  Screening of the 'Open Scaffolds' collection from Compounds Australia identifies a new chemical entity with anthelmintic activities against different developmental stages of the barber's pole worm and other parasitic nematodes.

Authors:  Sarah Preston; Yaqing Jiao; Jonathan B Baell; Jennifer Keiser; Simon Crawford; Anson V Koehler; Tao Wang; Moana M Simpson; Ray M Kaplan; Karla J Cowley; Kaylene J Simpson; Andreas Hofmann; Abdul Jabbar; Robin B Gasser
Journal:  Int J Parasitol Drugs Drug Resist       Date:  2017-05-28       Impact factor: 4.077

Review 4.  Anthelmintic drug discovery: target identification, screening methods and the role of open science.

Authors:  Frederick A Partridge; Ruth Forman; Carole J R Bataille; Graham M Wynne; Marina Nick; Angela J Russell; Kathryn J Else; David B Sattelle
Journal:  Beilstein J Org Chem       Date:  2020-06-02       Impact factor: 2.883

5.  Tetrahydroquinoxalines induce a lethal evisceration phenotype in Haemonchus contortus in vitro.

Authors:  Yaqing Jiao; Sarah Preston; Jose F Garcia-Bustos; Jonathan B Baell; Sabatino Ventura; Thuy Le; Nicole McNamara; Nghi Nguyen; Antony Botteon; Cameron Skinner; Jill Danne; Sarah Ellis; Anson V Koehler; Tao Wang; Bill C H Chang; Andreas Hofmann; Abdul Jabbar; Robin B Gasser
Journal:  Int J Parasitol Drugs Drug Resist       Date:  2018-12-30       Impact factor: 4.077

6.  High content analysis enables high-throughput nematicide discovery screening for measurement of viability and movement behavior in response to natural product samples.

Authors:  Jennifer M Petitte; Mary H Lewis; Tucker K Witsil; Xiang Huang; John W Rice
Journal:  PLoS One       Date:  2019-04-23       Impact factor: 3.240

7.  A suicide inhibitor of nematode trehalose-6-phosphate phosphatases.

Authors:  Megan Cross; Mark York; Ewa Długosz; Jan Hendrik Straub; Sonja Biberacher; H M P Dilrukshi Herath; Stephanie A Logan; Jeong-Sun Kim; Robin B Gasser; John H Ryan; Andreas Hofmann
Journal:  Sci Rep       Date:  2019-11-07       Impact factor: 4.379

8.  Anthelmintic activity of selected ethno-medicinal plant extracts on parasitic stages of Haemonchus contortus.

Authors:  Rasika Kumarasingha; Sarah Preston; Tiong-Chia Yeo; Diana S L Lim; Chu-Lee Tu; Enzo A Palombo; Jillian M Shaw; Robin B Gasser; Peter R Boag
Journal:  Parasit Vectors       Date:  2016-04-01       Impact factor: 3.876

Review 9.  Screening of the 'Pathogen Box' identifies an approved pesticide with major anthelmintic activity against the barber's pole worm.

Authors:  Sarah Preston; Yaqing Jiao; Abdul Jabbar; Sean L McGee; Benoît Laleu; Paul Willis; Timothy N C Wells; Robin B Gasser
Journal:  Int J Parasitol Drugs Drug Resist       Date:  2016-07-28       Impact factor: 4.077

10.  Screening of the 'Stasis Box' identifies two kinase inhibitors under pharmaceutical development with activity against Haemonchus contortus.

Authors:  Yaqing Jiao; Sarah Preston; Anson V Koehler; Andreas J Stroehlein; Bill C H Chang; Kaylene J Simpson; Karla J Cowley; Michael J Palmer; Benoît Laleu; Timothy N C Wells; Abdul Jabbar; Robin B Gasser
Journal:  Parasit Vectors       Date:  2017-07-05       Impact factor: 3.876
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