Su-Mi Woo1, Won-Jae Kim1, Hae-Soon Lim2, Nam-Ki Choi3, Sun-Hun Kim4, Seon-Mi Kim5, Ji-Yeon Jung6. 1. Department of Oral Physiology, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, South Korea; Research Center for Biomineralization Disorder, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, South Korea. 2. Department of Dental Education, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, South Korea. 3. Department of Pediatric Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, South Korea. 4. Research Center for Biomineralization Disorder, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, South Korea; Department of Oral Anatomy, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, South Korea. 5. Department of Pediatric Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, South Korea. Electronic address: impedo@jnu.ac.kr. 6. Department of Oral Physiology, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, South Korea. Electronic address: jjy@jnu.ac.kr.
Abstract
INTRODUCTION: Recent reports have shown that the combined use of platelet-rich fibrin (PRF), an autologous fibrin matrix, and mineral trioxide aggregate (MTA) as root filling material is beneficial for the endodontic management of an open apex. However, the potential of the combination of MTA and PRF as an odontogenic inducer in human dental pulp cells (HDPCs) in vitro has not yet been studied. The purpose of this study was to evaluate the effect of the combination of MTA and PRF on odontoblastic maturation in HDPCs. METHODS: HDPCs extracted from third molars were directly cultured with MTA and PRF extract (PRFe). Odontoblastic differentiation of HDPCs was evaluated by measuring the alkaline phosphatase (ALP) activity, and the expression of odontogenesis-related genes was detected using reverse-transcription polymerase chain reaction or Western blot. Mineralization formation was assessed by alizarin red staining. RESULTS: HDPCs treated with MTA and PRFe significantly up-regulated the expression of dentin sialoprotein and dentin matrix protein-1 and enhanced ALP activity and mineralization compared with those with MTA or PRFe treatment alone. In addition, the combination of MTA and PRFe induced the activation of bone morphogenic proteins (BMP)/Smad, whereas LDN193189, the bone morphogenic protein inhibitor, attenuated dentin sialophosphoprotein and dentin matrix protein-1 expression, ALP activity, and mineralization enhanced by MTA and PRFe treatment. CONCLUSIONS: This study shows that the combination of MTA and PRF has a synergistic effect on the stimulation of odontoblastic differentiation of HDPCs via the modulation of the BMP/Smad signaling pathway.
INTRODUCTION: Recent reports have shown that the combined use of platelet-rich fibrin (PRF), an autologous fibrin matrix, and mineral trioxide aggregate (MTA) as root filling material is beneficial for the endodontic management of an open apex. However, the potential of the combination of MTA and PRF as an odontogenic inducer in human dental pulp cells (HDPCs) in vitro has not yet been studied. The purpose of this study was to evaluate the effect of the combination of MTA and PRF on odontoblastic maturation in HDPCs. METHODS: HDPCs extracted from third molars were directly cultured with MTA and PRF extract (PRFe). Odontoblastic differentiation of HDPCs was evaluated by measuring the alkaline phosphatase (ALP) activity, and the expression of odontogenesis-related genes was detected using reverse-transcription polymerase chain reaction or Western blot. Mineralization formation was assessed by alizarin red staining. RESULTS: HDPCs treated with MTA and PRFe significantly up-regulated the expression of dentin sialoprotein and dentin matrix protein-1 and enhanced ALP activity and mineralization compared with those with MTA or PRFe treatment alone. In addition, the combination of MTA and PRFe induced the activation of bone morphogenic proteins (BMP)/Smad, whereas LDN193189, the bone morphogenic protein inhibitor, attenuated dentin sialophosphoprotein and dentin matrix protein-1 expression, ALP activity, and mineralization enhanced by MTA and PRFe treatment. CONCLUSIONS: This study shows that the combination of MTA and PRF has a synergistic effect on the stimulation of odontoblastic differentiation of HDPCs via the modulation of the BMP/Smad signaling pathway.
Authors: Sourabh R Joshi; Aparna U Palekar; Gowri S Pendyala; Viddyasagar Mopagar; Neeta Padmawar; Pratima Shah Journal: J Int Soc Prev Community Dent Date: 2020-08-06
Authors: Leopoldina D F Almeida; Pedro S Babo; Cristiana R Silva; Márcia T Rodrigues; Josimeri Hebling; Rui L Reis; Manuela E Gomes Journal: J Mater Sci Mater Med Date: 2018-06-14 Impact factor: 3.896