| Literature DB >> 2636262 |
S Schwarz1, M Cardoso, H Blobel.
Abstract
A small plasmid of 3.95 kb, encoding resistance to chloramphenicol (Cm) was detected in three of 33 Staphylococcus hyicus strains. The plasmid in each of the three strains was indistinguishable by Southern-blot hybridization and restriction enzyme analysis. It was shown by curing and by transformation to specify resistance to Cm. A preliminary restriction map of the plasmid, designated pSC2, is presented. Chloramphenicol acetyltransferase was demonstrated by enzyme assay and by SDS-PAGE of cell-free lysates of pSC2 transformants.Entities:
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Year: 1989 PMID: 2636262 DOI: 10.1099/00221287-135-12-3329
Source DB: PubMed Journal: J Gen Microbiol ISSN: 0022-1287