Yusuf Yucel1, Suzan Tabur2, Orhan Gozeneli1, Sezen Kocarslan3, Ahmet Seker1, Hasan Buyukaslan4, Emin Şavik5, Alpay Aktumen6, Abdullah Ozgonul1, Ali Uzunkoy1, Nurten Aksoy5. 1. a Department of General Surgery, Medical Faculty , Harran University , 63100 Sanliurfa , Turkey. 2. b Division of Endocrinology, Department of Internal Medicine , Gaziantep University Faculty of Medicine , Turkey. 3. c Department of Pathology, Medical Faculty , Harran University , 63100 Sanliurfa , Turkey. 4. d Emergency Department, Medical Faculty , Harran University , 63100 Sanliurfa , Turkey. 5. e Department of Clinical Biochemistry, Faculty of Medicine , Harran University , 63100 Sanliurfa , Turkey. 6. f Department of Pathology, Medical Faculty , Karabuk University , Karabuk , Turkey.
Abstract
OBJECTIVE: The aim of this study was to investigate the effects of lycopene (Lyc) on methotrexate (Mtx)-induced intestinal damage in rats. METHOD: Twenty-eight male Sprague Dawley rats were divided into four equal groups: control, Mtx, Lyc, and Mtx-L. CONTROL GROUP: Rats were given only the vehicle. Lyc group: Rats were given Lyc (10 mg/kg) with corn oil by oral gavage for 10 days. Mtx group: Rats were injected intraperitoneally with a single dose of 20 mg/kg of Mtx and given corn oil by oral gavage. Mtx-L group: Rats were treated with Lyc (10 mg/kg) for 10 days after a single dose of Mtx (20 mg/kg). All of the rats were euthanized using terminal anesthesia, and the intestinal tissues were removed for histological examination and for pro-inflammatory cytokine measurement (tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β)), total oxidative status (TOS), total antioxidant capacity (TAC), and oxidative stress index (OSI). RESULTS: Mtx administration increased histopathological damage and increased TNF-α, IL-1β, TOS, TAC, and OSI levels in the small intestine tissues. Lyc therapy applied to the Mtx-L group provided significant improvement in all parameters of histopathological damage to the small intestine and significantly reduced the levels of IL-1β, TOS, and OSI in the intestinal tissues. CONCLUSIONS: The results of this study indicate that Lyc might be useful for protecting intestinal damage induced by Mtx in rats by reducing the increased oxidative stress and pro-inflammatory cytokine (IL-1β) levels.
OBJECTIVE: The aim of this study was to investigate the effects of lycopene (Lyc) on methotrexate (Mtx)-induced intestinal damage in rats. METHOD: Twenty-eight male Sprague Dawley rats were divided into four equal groups: control, Mtx, Lyc, and Mtx-L. CONTROL GROUP: Rats were given only the vehicle. Lyc group: Rats were given Lyc (10 mg/kg) with corn oil by oral gavage for 10 days. Mtx group: Rats were injected intraperitoneally with a single dose of 20 mg/kg of Mtx and given corn oil by oral gavage. Mtx-L group: Rats were treated with Lyc (10 mg/kg) for 10 days after a single dose of Mtx (20 mg/kg). All of the rats were euthanized using terminal anesthesia, and the intestinal tissues were removed for histological examination and for pro-inflammatory cytokine measurement (tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β)), total oxidative status (TOS), total antioxidant capacity (TAC), and oxidative stress index (OSI). RESULTS:Mtx administration increased histopathological damage and increased TNF-α, IL-1β, TOS, TAC, and OSI levels in the small intestine tissues. Lyc therapy applied to the Mtx-L group provided significant improvement in all parameters of histopathological damage to the small intestine and significantly reduced the levels of IL-1β, TOS, and OSI in the intestinal tissues. CONCLUSIONS: The results of this study indicate that Lyc might be useful for protecting intestinal damage induced by Mtx in rats by reducing the increased oxidative stress and pro-inflammatory cytokine (IL-1β) levels.
Authors: Murat Rabus; Recep Demirbağ; Yusuf Sezen; Oğuz Konukoğlu; Ali Yildiz; Ozcan Erel; Rahmi Zeybek; Cevat Yakut Journal: Turk Kardiyol Dern Ars Date: 2008-12
Authors: Marwa Ahmed Abass; Shereen Ahmed Elkhateeb; Samia Adel Abd El-Baset; Asmaa Alhosiny Kattaia; Eman Mosallam Mohamed; Hebatallah Husseini Atteia Journal: Environ Sci Pollut Res Int Date: 2016-04-22 Impact factor: 4.223