Martin Weiss1, Denis Gümbel2, Nadine Gelbrich2, Lars-Ove Brandenburg3, Robert Mandelkow1, Uwe Zimmermann1, Patrick Ziegler4, Martin Burchardt1, Matthias B Stope5. 1. Department of Urology, University Medicine Greifswald, Greifswald, Germany. 2. Department of Trauma and Orthopedic Surgery, University Medicine Greifswald, Greifswald, Germany. 3. Department of Anatomy and Cell Biology, RWTH Aachen Universtity, Aachen, Germany. 4. Institute for Occupational and Social Medicine, RWTH Aachen Universtity, Aachen, Germany. 5. Department of Urology, University Medicine Greifswald, Greifswald, Germany matthias.stope@uni-greifswald.de.
Abstract
UNLABELLED: Backround/Aim: Physical plasmas are ionized gases containing several biologically-reactive factors that yet exert their anti-microbial and anti-proliferative effects in fields of surface sterilisation, de-contamination and wound healing. MATERIALS AND METHODS: Cold atmospheric plasma (CAP) was generated via the atmospheric pressure plasma jet kINPen09. Apoptotic effects of CAP treatment on the human epithelial prostate cancer cell line LNCaP as a cell culture model for malignant tumor tissue was analyzed by cell counting, western blot and quantitative reverse transcription-polymerase chain reaction analysis. RESULTS: LNCaP cells exhibited significantly reduced cell growth following CAP treatment. We show that most probably the induction of apoptosis is the terminus of CAP treatment illustrated by the pro-apoptotic modulation of p53, p21, caspase-3, Bax, and survivin, as well as morphological changes of cell architecture. CONCLUSION: Our in vitro study offers first indicatory results for molecular response mechanisms after CAP treatment in a suitable LNCaP cell model.
UNLABELLED: Backround/Aim: Physical plasmas are ionized gases containing several biologically-reactive factors that yet exert their anti-microbial and anti-proliferative effects in fields of surface sterilisation, de-contamination and wound healing. MATERIALS AND METHODS: Cold atmospheric plasma (CAP) was generated via the atmospheric pressure plasma jet kINPen09. Apoptotic effects of CAP treatment on the humanepithelial prostate cancer cell line LNCaP as a cell culture model for malignant tumor tissue was analyzed by cell counting, western blot and quantitative reverse transcription-polymerase chain reaction analysis. RESULTS: LNCaP cells exhibited significantly reduced cell growth following CAP treatment. We show that most probably the induction of apoptosis is the terminus of CAP treatment illustrated by the pro-apoptotic modulation of p53, p21, caspase-3, Bax, and survivin, as well as morphological changes of cell architecture. CONCLUSION: Our in vitro study offers first indicatory results for molecular response mechanisms after CAP treatment in a suitable LNCaP cell model.
Authors: Thomas Wenzel; Daniel A Carvajal Berrio; Christl Reisenauer; Shannon Layland; André Koch; Diethelm Wallwiener; Sara Y Brucker; Katja Schenke-Layland; Eva-Maria Brauchle; Martin Weiss Journal: Cancers (Basel) Date: 2020-01-22 Impact factor: 6.639
Authors: Lyubomir Haralambiev; Lasse Wien; Nadine Gelbrich; Jörn Lange; Sinan Bakir; Axel Kramer; Martin Burchardt; Axel Ekkernkamp; Denis Gümbel; Matthias B Stope Journal: Oncol Lett Date: 2019-11-19 Impact factor: 2.967